The invention relates to a hydrological model parameter calibration method based on multiple hydrological variables, and the method comprises the following steps: S1, obtaining long-time series hydrometeorological data, remote sensing inversion soil humidity and evaporation capacity data in a watershed range, and taking one year as a minimum unit, dividing the data period into a plurality of different sub-periods according to the conditions of annual precipitation, annual runoff and drainage basin underlying surface; s2, aiming at each sub-period, on the basis of remote sensing inversion evaporation capacity data and soil humidity index SWI data, calibrating hydrological model parameters in two modes of joint calibration and step-by-step calibration; and S3, by taking the King-Gupta efficiency coefficient as an evaluation index, analyzing and evaluating the flow process simulation effect of the model parameters in each rate stator sub-period and the flow process simulation effect of the model parameters transferred to other sub-periods. According to the method, the uncertainty of the model parameters can be effectively reduced, the mobility of the model parameters in different time periods is improved, and the reliability of the watershed hydrological model in production practice can be improved.