The invention discloses an estimation method for a plurality of Doppler frequency offsets of an OFDM (orthogonal frequency division multiplexing) system in a high-speed mobile environment. The estimation method comprises the following steps that: a mobile relay terminal is set on a high-speed mobile vehicle and used for forwarding communication data between a base station and a mobile station, at the time, transmission routes with a limited number exist between the base station and the mobile relay terminal, and each route corresponds to different Doppler frequency offsets; and a plurality of Doppler frequency offsets between the base station and the mobile station on the vehicle are obtained by analysing the plurality of Doppler frequency offsets between the base station and the mobile relay terminal. With the adoption of the method disclosed by the invention, estimation for the plurality of Doppler frequency offsets in the high-speed mobile environment is realized. Moreover, the greater a sub-carrier number Nc is, the smaller a mean square error under the same signal-to-noise ratio is, namely, the more accurate the estimation for the frequency offsets is. Because in the case that Nb is given, increase for the Nc is increase for Na essentially, and the Na is equal to the fast beat number of a signal, the accuracy of estimation for a covariance matrix Rd can be improved via the increase for the Nc, thus the estimation for the frequency offsets is more accurate.