The invention belongs to the field of medical materials and relates to a lithium disilicate glass ceramic, a preparation method thereof and application. The lithium disilicate glass ceramic is prepared from, by mass, SiO2 55%-65%, Li2O 16%-24%, P2O52 2%-5%, Al2O3 8%-12%, K2O 2%-5% and TbO2 1%-10%. According to the method, raw materials are subjected to pre-sintering, calcination, molding by casting and then are subjected to annealing, coring and crystallization to prepare a lithium metasilicate glass ceramic block, the lithium metasilicate glass ceramic block has very good machinability, and heat treatment is performed again after machining to obtain the lithium disilicate glass ceramic. The lithium disilicate glass ceramic prepared by the preparation method is doped with a specific content of rare earth element Tb, a glass grid structure can be destroyed, the viscosity can be reduced, crystallization is promoted, the hardness and bending strength of the glass ceramic are improved, and a novel method is provided for preparation of the lithium disilicate glass ceramic.