This invention provides a method, apparatus, electronic device, and storage medium for determining the Tm value of a melting curve, relating to the field of PCR detection technology. In determining the Tm value, this invention first obtains the initial melting curve for each well of a PCR plate; then, based on the
wavelet modulus maxima peak-finding principle of
continuous wavelet transform, it searches for peaks in the initial melting curve to obtain the initial Tm value; finally, it uses a fixed threshold method to denoise and filter the initial Tm value to obtain the target Tm value. This method does not require any
smoothing filtering, avoiding the sensitivity to
noise and
background information problems of conventional melting curve Tm value
calculation methods, and is easy to understand and implement.