2 results about "WNT5A" patented technology

This invention proposes the application of a reagent for detecting biomarkers in the preparation of a kit for diagnosing asthma. The biomarkers of this invention are any one or more of PDK4, TM4SF1, WIF1, and WNT5A. The reagent for detecting biomarkers of this invention is used to detect the expression level and / or methylation level of the biomarker in a sample. It can be used in a kit for diagnosing asthma, enabling early and accurate diagnosis of asthma with excellent diagnostic efficacy. This allows for the screening of drugs for the prevention or treatment of asthma, solving the problems of inaccurate judgment and inconvenience in traditional techniques that rely on clinical manifestations, pulmonary function tests, or peak flow rate measurements for asthma diagnosis. This fills a gap in asthma diagnostic kits and provides a fast and convenient method for asthma diagnosis.

The application discloses application of WNT5A gene in regulation and control of host resistance to bovine parainfluenza virus type 3 (BPIV-3). By using CRISPR / Cas9 gene editing technology, a WNT5A gene knockout MDBK cell line and a BPIV-3 infection resistant cell model are constructed, it is found that the gene knockout cell shows significant resistance to BPIV-3 infection, the proliferation speed of BPIV-3 on the gene knockout cell is significantly lower than that of the wild type cell, and the complementation of the WNT5A gene leads to the disappearance of the antiviral effect of the cell. The above results show that the WNT5A gene plays an important role in regulation and control of host resistance to virus. The application identifies the antiviral regulation function of the WNT5A gene at the cell level, for the first time finds that the gene can be used in resistance breeding and research and development of prevention and treatment drugs for bovine BPIV-3 infection as a potential target point for gene editing cell and animal design, and has great significance for reducing drug abuse and avoiding epidemic occurrence.