This invention proposes the application of a
reagent for detecting biomarkers in the preparation of a kit for diagnosing
asthma. The biomarkers of this invention are any one or more of PDK4, TM4SF1, WIF1, and WNT5A. The
reagent for detecting biomarkers of this invention is used to detect the expression level and / or
methylation level of the biomarker in a sample. It can be used in a kit for diagnosing
asthma, enabling early and accurate diagnosis of
asthma with excellent diagnostic
efficacy. This allows for the screening of drugs for the prevention or treatment of asthma, solving the problems of inaccurate judgment and inconvenience in traditional techniques that rely on clinical manifestations, pulmonary function tests, or peak flow rate measurements for asthma diagnosis. This fills a gap in asthma diagnostic kits and provides a fast and convenient method for asthma diagnosis.