5 results about "WNT5A" patented technology

The invention discloses a predictive marker as well as a related product and application thereof, and relates to the field of tumor diagnosis. The invention finds that WNT5A can be used as a marker of any one or more of disease risk, prognosis risk, disease progress, treatment effect, residual focus and metastatic focus of squamous-cell carcinoma, is beneficial to accurate diagnosis and treatment of squamous-cell carcinoma, and accelerates the research and development of new drugs.

This invention proposes the application of a reagent for detecting biomarkers in the preparation of a kit for diagnosing asthma. The biomarkers of this invention are any one or more of PDK4, TM4SF1, WIF1, and WNT5A. The reagent for detecting biomarkers of this invention is used to detect the expression level and / or methylation level of the biomarker in a sample. It can be used in a kit for diagnosing asthma, enabling early and accurate diagnosis of asthma with excellent diagnostic efficacy. This allows for the screening of drugs for the prevention or treatment of asthma, solving the problems of inaccurate judgment and inconvenience in traditional techniques that rely on clinical manifestations, pulmonary function tests, or peak flow rate measurements for asthma diagnosis. This fills a gap in asthma diagnostic kits and provides a fast and convenient method for asthma diagnosis.

The invention provides an anti-ROR1 nano antibody and an application thereof. Through alpaca immunization and a phage library, a high-affinity nano antibody HCAbs1 is screened out, and through flow cytometry and a pull-down experiment, it is determined that the HCAbs1 can be effectively combined with ROR1. The HBABs1 can reduce the ERK phosphorylation level stimulated by Wnt5a in ROR1 stably transfected cells, and has huge potential in the aspects of cancer diagnosis and treatment.

The invention provides a nerve cannula loaded with a small molecule preparation Pyrvinium pamoate, a preparation method and application, belongs to the technical field of biological materials, and solves the bottleneck problems that Wnt5a as macromolecular protein is difficult to realize clinical transformation and is difficult to jointly promote peripheral nerve injury repair from multiple aspects. The preparation method of the nerve cannula comprises the following steps: step 1, preparing the nerve cannula; step 2, preparing a DNA (Deoxyribose Nucleic Acid) hydrogel for slowly releasing the Pyrviniumpamoate; 3, the DNA hydrogel containing the Pyrvinumpamoate prepared in the step 2 is sucked into a microsyringe, the DNA hydrogel containing the Pyrvinumpamoate is injected into a nerve socketed pipe, and the nerve socketed pipe loaded with the Pyrvinumpamoate is obtained. A key receptor Vangl2 of a Wnt5a signal channel is regulated and controlled through a Vangl2 agonist (Pyrvinumpamoate), and the small molecule agonist is loaded through a nerve socketed tube to play a role in repairing peripheral nerve defects, so that the advantage of the Wnt5a for jointly promoting peripheral nerve injury repair from three aspects of axon regeneration, myelination and vascular regeneration is fully played.

The application discloses application of WNT5A gene in regulation and control of host resistance to bovine parainfluenza virus type 3 (BPIV-3). By using CRISPR / Cas9 gene editing technology, a WNT5A gene knockout MDBK cell line and a BPIV-3 infection resistant cell model are constructed, it is found that the gene knockout cell shows significant resistance to BPIV-3 infection, the proliferation speed of BPIV-3 on the gene knockout cell is significantly lower than that of the wild type cell, and the complementation of the WNT5A gene leads to the disappearance of the antiviral effect of the cell. The above results show that the WNT5A gene plays an important role in regulation and control of host resistance to virus. The application identifies the antiviral regulation function of the WNT5A gene at the cell level, for the first time finds that the gene can be used in resistance breeding and research and development of prevention and treatment drugs for bovine BPIV-3 infection as a potential target point for gene editing cell and animal design, and has great significance for reducing drug abuse and avoiding epidemic occurrence.