The present invention discloses a construction method for a compound
thrombus clearing preparation bioactivity
chromatography finger print. The construction method comprises the following steps: (1) preparing a compound
thrombus clearing preparation
test solution, precisely sucking 10 mul of the compound
thrombus clearing preparation
test solution, and injecting into a double-pump dual-gradient high performance liquid chromatograph to obtain a compound thrombus clearing preparation HPLC
finger print; (2) preparing a mixed reference substance solution, precisely sucking 10 mul of the reference substance solution, and injecting into the double-pump dual-gradient high performance liquid chromatograph to obtain a reference substance HPLC
finger print; (3) adopting a reference
substance control method, and adopting an RRLC / MS / MS technical method to prove 21 chemical components in the finger print; and (4) analyzing correlation between the 21 chemical components and bioactivity difference, clarifying bioactivity component groups, and establishing a compound thrombus clearing preparation bioactivity
chromatography finger print. According to the present invention, the compound thrombus clearing preparation HPLC finger print and the bioactivity component groups obtained from chromatograph efficiency analysis are combined to construct the compound thrombus clearing preparation bioactivity
chromatography finger print so as to completely monitor safeties, effectiveness and quality uniformities of
raw material herbs, semi-finished products and finished products.