The invention provides a mobile phone and computer anti-radiation eye protection liquid. The eye protection liquid is prepared from the following components in parts by weight: 1 to 2 parts of ofloxacin eye drops, 0.3 to 0.8 part of aloe vera, 0.2 to 0.6 part of Shangnong large leaf aloe, 0.4 to 0.9 part of aloe arborescens; 0.4-1 part of cactus, 0.4 to 0.8 part of hylocereus undatus; 0.2-0.5 partof ball cactus, 0.5-1 part of homalomena occulta, 1-2 parts of palm flowers, and 0.5-1 part of succulent plants. The preparation method of the eye protection liquid comprises the following steps: S1,selecting the plant materials, cleaning, and adding some diluted plant disinfectants to carry out a disinfection treatment. The eye protection liquid has the advantages that the raw materials are abundant, the production process is simple, the application of the eye protection liquid is convenient; the eye protection liquid is uniformly smeared on a mobile phone/computer screen, a protective filmis pasted after the liquid is slightly dried, the radiation of a mobile phone and a computer to eyes can be effectively reduced after the eye protection liquid is used, damage to the eyes is reduced,and the eye protection liquid is worthy of large-scale application and popularization.