Methods and apparatuses for target
particle separation and isolation are disclosed. The apparatuses are flow chambers that include an infusion port configured to introduce a sample, and an
elution flow port configured to receive an
elution buffer. The flow chamber also includes an
electrode coupled to a floor of the flow chamber, wherein the
electrode includes at least a first zone with a first inter-
electrode pitch and a second zone with a second inter-electrode
pitch. The first zone generates a first
electric field and the second zone generates a second
electric field of a different magnitude than the first
electric field when an
electric signal is applied. The first electric field and the second electric field separate the target particle from the fluidic suspension via
dielectrophoresis. The flow chamber also includes a
waste disposal port that discharges the
waste material, and a collection port configured that collects a target particle enriched sample.