The invention provides a land use partition method based on tabu search algorithm, aiming to solve the problem that the traditional land use space partition method hardly takes multi-target characteristics of land use into consideration and lacks intelligent and efficient partition decision algorithm. The tabu search algorithm in mathematics field is applied in the land use space partition field; and key issues such as initial plan generation method, neighborhood rule definition, neighborhood generation method, neighborhood search method, memory list update, breadth search, depth search and restarting strategy are modified, a technical method for supporting the land use partition optimization decision is provided, the problem for optimizing spatial unit combination of multi-target in the land use partition is solved better and the problem of hard constraint conditions such as the continuity and integrity of spatial district is considered, so that the partition efficiency is higher and the stability and high efficiency of land use partition process are guaranteed.