The invention discloses a multi-threshold image segmentation method based on a crossover mutation artificial fish swarm algorithm and mainly aims to solve the problem that in the prior art, information loss of a segmented image is serious. The method comprises the implementation steps that 1, an image is input, and pixel grayscale values at all image pixel points are acquired; 2, c thresholds are selected to segment the image into c+1 classes; 3, n artificial fishes are generated, and each artificial fish is a 1xc-dimension vector and represents a group of threshold possible solutions; 4, a fitness function made according to the kapur maximum entropy criterion is regarded as a goal, and a maximum value of the fitness function is searched for; and 5, a group of thresholds corresponding to the fitness maximum value found through search are utilized to perform image segmentation, the pixel points with the grayscale values in the same interval are classified into one class, and the segmented image is output. Through the method, the optimizing precision of the artificial fish swarm algorithm in the optimizing process is effectively improved, the image segmentation effect is improved further in combination with multi-threshold image segmentation, and the method can be applied to computer vision analysis.