The invention discloses a preparation method of a
growth factor gradient bionic
scaffold for
tendon-bone interface regeneration and repair, and belongs to the technical field of biomedical materials. The
composite scaffold is constructed by adopting a
coaxial electrospinning and self-
assembly technology, and is compounded by a core-shell oriented
nanofiber layer and a non-oriented
nanofiber layer through a
gelatin adhesive to respectively simulate
tendon phase and bone phase structures of a
tendon-bone interface; a shell layer of the tendon-phase-simulated core-shell oriented
nanofiber is a PCL layer containing
CTGF, a core layer of the tendon-phase-simulated core-shell oriented nanofiber is a PVA layer containing TGF-beta1, and a bone-phase-simulated non-oriented nanofiber layer is a PCL / Gel co-spun layer containing BMP-2
polylactic acid microspheres and nano-hydroxyapatite. The
scaffold can realize secondary
controlled release of early-stage release of
CTGF, slow release of TGF-beta1 in the middle and later periods and
continuous release of BMP-2, and cooperates with a
fiber orientation structure to induce graded differentiation of mesenchymal stem cells and promote integrated repair of a tendon-bone interface gradient structure, so that the problems of poor healing of a tendon-bone interface and high repeated fracture rate in a traditional
repair method are solved, and the
scaffold has good application prospects. The important application value is realized in the field of tendon-
bone injury treatment in
sports medicine.