This application relates to the field of
molecular pathology diagnostic technology, specifically disclosing a
bone marrow smear FISH detection kit and its application. The kit includes a rapid hybridization buffer, a stabilization pretreatment solution,
room temperature hybridization probes, probe
dilution buffer, low-salt washing buffer, high-salt washing buffer, counterstaining solution,
positive control samples, and
negative control samples. The
room temperature hybridization probes include broken probe pairs targeting the BCR-
ABL, PML-RARA fusion genes, and IgH
gene rearrangements. The detection method of this kit includes sample pretreatment, probe denaturation,
room temperature hybridization, washing and counterstaining, and
signal interpretation. Through the synergistic effect of the rapid hybridization buffer and the short-chain low-Tm value probes, rapid hybridization at room temperature is achieved, eliminating the need for a dedicated isothermal hybridization instrument. The stabilization pretreatment solution can complete
cell permeation and
RNA removal in one step, resulting in a high degree of operational
standardization.