The invention belongs to the technical field of criminal investigation teaching, and discloses a virtual simulation practical training teaching application system and method for criminal investigation. The virtual simulation practical training teaching application system comprises a student login module, a virtual experiment module, an experiment starting module, a crime scene investigation module, an investigating and visiting module, a case detail analysis module, a technical investigation module, a physical evidence technology module and an inquiry module. Through the student login module,a student inputs a correct user name and a correct password to log in the system; through the virtual experiment module, the type of a to-be-conducted virtual simulation experiment is selected througha virtual simulation experiment list; through the experiment starting module, after experiment starting is clicked, the student enters a commanding center virtual scene, and case reporting alarm receiving is simulated; through the crime scene investigation module, alert block is conducted on a crime scene, and physical evidences are extracted and stored; through the investigating and visiting module, people around the crime scene are investigated and visited; through the case detail analysis module, professional analysis is conducted on case details in a virtual commanding center conference room. By conducting virtual simulation on the processing procedures of criminal investigation, the student can be taught more visually, and the effect of training is improved.