The invention discloses a soft capsule rich in xanthophylls and multiple vitamins and a preparation method of the soft capsule. The soft capsule is characterized by comprising a capsule content and a capsule wall material, wherein the capsule content comprises the following components in percentage by weight: 1-3 percent of xanthophylls, 0.02-0.04 percent of vitamin A, 3-5 percent of vitamin C, 1-3 percent of vitamin E, 3-5 percent of zinc gluconate, 1-3 percent of beewax and 85-90 percent of linseed oil. The preparation method comprises the following steps of: weighing required xanthophylls, vitamin A, vitamin C, vitamin E, zinc gluconate, beewax and linseed oil according to the quantity of the formula, adding in the components in a ball grinding machine, grinding uniformly; respectively weighing gelatin, glycerol and purified water according to the proportion of 1:0.4:1 in weight, dissolving the gelatin in a gelatin dissolving device, adding 0.3-0.5 percent of officinal red iron oxide, uniformly stirring; and loading the prepared capsule content and the capsule wall material in a pelleting machine for pelleting, sizing and drying to obtain the soft capsule with required specification. The invention has the important contribution of using linseed oil efficiently protecting vision as a matrix, thus the functions of alleviating asthenopia and improving vision are greatly enhanced.