The invention discloses amino acid fertilizer water slurry special for aquaculture and a preparation method of the amino acid fertilizer water slurry. The amino acid fertilizer water slurry consists of amino acid solution, microelements, fermentation organic matters, inorganic fertilizer, mineral matters, a water quality modifier and beneficial bacteria according to a certain proportion. The preparation method comprises the following steps of: A, producing the amino acid solution, i.e., a, hydrolyzing, b, neutralizing, c, filtering and d, concentrating; B, producing the fermentation organic matters, i.e., a, stirring, uniformly stirring organic matter raw materials with the amino acid solution, b, inoculating, uniformly stirring the organic matter raw materials with the beneficial bacteria, and c, fermenting; and C, producing the amino acid fertilizer water slurry, i.e., a, chelating, heating the amino acid solution, and adding the microelements under stirring for chelating, b, mixing, adding the fermentation organic matters, the inorganic fertilizer, the mineral matters, the water quality modifier and the beneficial bacteria in sequence and uniformly stirring and mixing, c, checking according to related quality standard, and d, packaging. According to the amino acid fertilizer water slurry, the immunity of fishes is improved, diseases are reduced, the breeding yield of spotted sliver carp is increased, the fish breeding cost is reduced, and the fish quality is improved.