The present invention generally relates to devices and methods for the preservation of cells using
drying, freezing, and other related techniques. In one set of embodiments, the invention allows for the preservation of cells in a dried state. In another set of embodiments, the invention allows for the preservation of cells within a glass or other non-viscous, non-frozen media. In some embodiments, the invention allows for the preservation of cells at temperatures below the
freezing point of water, and in some cases at cryogenic temperatures, without inducing
ice formation. The cells, in certain embodiments, may be preserved in the presence of
intracellular and / or
extracellular carbohydrates (which may be the same or different), for example,
trehalose and
sucrose. Carbohydrates may be transported intracellularly by any suitable technique, for example, using
microinjection, or through non-microinjected methods such as through pore-forming proteins,
electroporation,
heat shock, etc. In certain instances, the
glass transition temperature of the cells may be raised, e.g., by transporting a
carbohydrate intracellularly. In some cases, the cells may be dried and / or stored, for example, in a substantially
moisture-saturated environment or a desiccating environment. The cells may also be stored in a vacuum or a partial vacuum. The cells may be protected from
oxygen,
moisture, and / or light during storage. In certain cases, an inhibitor, such as a
cell death inhibitor, a
protease inhibitor, an
apoptosis inhibitor, and / or an
oxidative stress inhibitor may be used during preservation of the cells. The cells may be stored for any length of time, then recovered to a viable state, e.g., through rehydration, for further use.