53 results about "Gladiolus hybridus" patented technology

The invention relates to the technical field of microorganisms, and discloses burkholderia gladioli as well as a bacterial agent and application of the burkholderia gladioli. The Burkholderia gladioli (Burkholderia gladioli) CCTCC (China Center For Type Culture Collection) NO: M20242288 provided by the invention has good capacities of fixing nitrogen, dissolving phosphorus, dissolving zinc, producing siderophores and oxidizing sulfur, and can be used for regulating the nutrient structure of soil and converting substances which are difficult to utilize by plants in the soil, so that the growth of the plants is promoted, and the stress resistance of the plants is improved; the strain also can be used for antagonizing Fusarium solani, Fusarium equiseti, Fusarium verticillium, Fusarium verticillium and Rosellinia necatrix, can be used for antagonizing Fusarium solani, Fusarium equiseti, Fusarium verticillium and Rosellinia necatrix, and can also be used for antagonizing Fusarium solani, Fusarium equiseti, Fusarium verticillium, Rosellinia necatrix, Fusarium moniliforme and Rosellinia necatrix, Fusarium moniliforme and Rosellinia necatrix. Besides, the burkholderia gladioli provided by the invention is a strain separated from rape farmland soil, and compared with a strain screened by extreme environment and laboratory mutagenesis, the burkholderia gladioli has better adaptability when being applied to a planting environment, and can be stably colonized in plant rhizosphere and soil, so that the burkholderia gladioli stably plays a growth promoting role for a long time, is safe and environment-friendly, and has a wide application prospect. Wide application prospects are realized.

The invention discloses a Burkholderia gladioli (Burkholderia gladioli) CT-5 strain and an application of the Burkholderia gladioli CT-5 strain, and relates to the technical field of microorganisms. The strain is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M20242732, and the preservation date is December 05, 2024. The Burkholderia gladioli (Burkholderia gladioli) CT-5 provided by the invention has a remarkable prevention and treatment effect (Plt, Plt) on idesia root rot caused by Fusarium solani (Fusarium solani), and the Burkholderia gladioli (Burkholderia gladioli) CT-5 has a remarkable prevention and treatment effect on idesia root rot caused by Fusarium solani (Busarium solani). The compound has the advantages that the compound can be used for inhibiting pathogenic bacteria of various plants, and the compound can be used for inhibiting pathogenic bacteria of various plants, such as Schizophyllum commune, Diaporthe erens, Colletotrichum acutatum and the like, and has a remarkable inhibiting effect on other various plant pathogenic bacteria such as the Schizophyllum commune, the Diaporthe erens, the Colletotrichum acutatum and the like. In a pot experiment, the biomass, soil nutrients, SOD, POD and CAT enzyme activities of idesia polycarpa seedlings in a CT-5 treatment group are remarkably improved compared with those of a pathogenic bacteria control group (FP) (Plt; 0.05), which indicates that the strain can promote plant growth and activate the activity of defensive enzyme in the body, so that effective control of idesia root rot is realized, and powerful theoretical basis and technical support are provided for sustainable development of idesia.

The invention discloses burkholderia gladioli and an application of the burkholderia gladioli. The invention provides a Burkholderia gladioli HZX-8 strain, and the preservation number of the Burkholderia gladioli HZX-8 strain is CGMCC (China General Microbiological Culture Collection Center) No.33009. The Burkholderia gladioli HZX-8 strain has the advantages that the The invention also provides an application of the strain or a culture solution or a bacterial solution or a fermentation culture solution of the strain in preventing or treating plant walnut anthracnose and / or walnut branch blight. The Burkholderia gladioli HZX-8 strain disclosed by the invention has the advantages that the Burkholderia gladioli HZX-8 strain obtained by the invention shows a remarkable biological control potential in the aspects of preventing and treating the walnut anthracnose caused by the Colletotrichum guangyuanense and the walnut branch blight caused by the Diapotheca chaotianensis, and the Burkholderia gladioli HZX-8 strain can be used for preventing and treating the walnut branch blight caused by the Colletotrichum guangyuanense and the Burkholderia gladioli HZX-8 strain can be used for preventing and treating the walnut branch blight caused by the Diapotheca chaotianense.

The invention discloses Burkholderia gladioli HM-72 and application thereof, the Burkholderia gladioli HM-72 is preserved in China Center for Type Culture Collection on July 13, 2023, the preservation address is Wuhan University, Wuhan, No. 299, eight road, Wuchang District, Wuhan, Hubei, and the preservation number is CCTCC NO: M20231291. The strain is high in Pb stress resistance, and has the capability of partially dissolving phosphorus and producing siderophores. After the strain is applied to the Pb-polluted soil, the migration rate of Pb can be improved, the removal of Pb in the soil is accelerated, the Pb is accumulated in the overground part of the enrichment plant, the problem of repairing the lead pollution of the soil is solved, meanwhile, the strain can inhibit the growth of pathogenic fungi, the growth promoting and bacteriostasis characteristics of the strain can provide favorable conditions for the growth of the enrichment plant in a high-lead environment, and the application prospect is wide. The biomass accumulation is promoted, and the method has an application prospect in heavy metal polluted environment restoration.

The invention discloses burkholderia gladioli and application of the burkholderia gladioli in prevention and treatment of grape diseases, the classification name of the burkholderia gladioli is burkholderia gladioli, the burkholderia gladioli is preserved in China General Microbiological Culture Collection Center on April 1, 2024, the preservation number is CGMCC No.30228, and the address of the preservation unit is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain is wide in antibacterial spectrum, and has good antibacterial activity on common pathogenic bacteria in grape planting, especially on grape white rot and grape anthracnose. According to the research, through directional screening of grape rhizosphere soil microorganisms, biocontrol bacteria capable of efficiently antagonizing grape white rot bacteria are pre-obtained, and a basic material is provided for biological prevention and control of grape white rot.

The invention relates to a body temperature amplification test strip rapid detection method of Burkholderia gladioli coconut poison pathogenic variants in food. The invention also relates to an oligonucleotide primer probe composition for the method. The invention also relates to a detection kit containing the composition. When the composition is used for test strip body temperature amplification detection, burkholderia gladioli coconut poison pathogenic variant components in a food sample can be simply, rapidly, specifically and sensitively detected.

The invention discloses burkholderia gladioli BG-GF5 as well as a composition and application thereof, the burkholderia gladioli BG-GF5 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: M20242573, and the preservation date is November 25, 2024. The application of the BG-GF5 is mainly reflected in the inhibition effect of the BG-GF5 on pathogenic bacteria and the potential of the BG-GF5 in crop disease control. The strain or the fermentation product and the metabolite thereof can be prepared into a composition for inhibiting pathogenic bacteria and crop diseases caused by the pathogenic bacteria. Particularly, the application to crops such as bee sugar plums, chilies and kiwi fruits shows a wide application prospect in the field of agricultural biological control.

A piece of natural plant material is subjected to one or more chemical treatments to remove substantially all lignin therefrom, thereby allowing the extraction of delignified, cellulose-based fibers. For example, the natural plant material can be a grass, such as bamboo or gladiolus. Subsequent drying of the extracted fiber densifies the structure, yielding improved mechanical properties. In some embodiments, the extracted fibers can be used, either alone or in combination with other materials, as a structural material. For example, the extracted fibers can be embedded within, infiltrated with, coated by, or otherwise combined with a polymer or concrete to form a composite material.

The application discloses a strain of Burkholderia gladioli CT-5 and application thereof, and relates to the technical field of microorganisms. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M20242732, and the preservation date is December 5, 2024. The Burkholderia gladioli CT-5 provided by the application has a significant prevention and treatment effect (P<0.05) on root rot of Idesia polycarpa caused by Fusarium solani, and has a significant inhibitory effect on other various plant pathogenic fungi such as Schizophyllum commune, Diaporthe eres and Colletotrichum acutatum. In a pot experiment, the biomass of Idesia polycarpa seedlings, soil nutrients, SOD, POD and CAT enzyme activities of the CT-5 treatment group are significantly improved (P<0.05) compared with the pathogenic fungus control group (FP), indicating that the strain can promote plant growth, activate the activity of defense enzymes in the body, and thus effectively control the root rot of Idesia polycarpa, which provides strong theoretical basis and technical support for the sustainable development of Idesia polycarpa.

The present invention relates to the technical field of bacteriophages, and provides a bacteriophage for lysing Burkholderia gladioli and a use of the bacteriophage. The provided bacteriophage for lys

The application discloses a Burkholderia gladioli BG-GF5, a composition and application thereof. The strain is preserved in the China Center for Type Culture Collection with a preservation number of CCTCC NO: M20242573, and the preservation date is November 18, 2024. The application of the BG-GF5 mainly reflects the inhibition effect on pathogenic bacteria and the potential in crop disease control. The strain or the fermentation product or the metabolic product thereof can be made into a composition for inhibiting pathogenic bacteria and crop diseases caused by pathogenic bacteria. Especially in the application of crops such as honey plum, pepper and kiwi, the application shows a wide application prospect in the field of agricultural biological control.

The invention relates to the technical field of microorganisms, and discloses a burkholderia gladioli bacteriophage and application thereof. Wherein the preservation number of the burkholderia gladioli bacteriophage is CGMCC (China General Microbiological Culture Collection Center) No.45883. The bacteriophage provided by the invention has a very strong lysis effect on burkholderia gladioli, can be used for preventing and controlling burkholderia gladioli, such as corn diseases caused by burkholderia gladioli, and has a good application prospect. Meanwhile, the bacteriophage provided by the invention can enlarge a bacteriophage resource library of burkholderia gladioli, so that diseases caused by burkholderia gladioli can be more comprehensively prevented and treated.

The application discloses a Burkholderia gladioli and application thereof in prevention and treatment of grape diseases, the classification and naming of the bacteria is Burkholderia gladioli, which is preserved in the China General Microbiological Culture Collection Center on April 1, 2024, and the preservation number is CGMCC No.30228, and the address of the preservation unit is No.3, Xili, Beichen West Road, Chaoyang District, Beijing City. The strain has a wide antibacterial spectrum, and has good antibacterial activity on common pathogenic bacteria in grape planting, especially on grape white rot and grape anthracnose. Through directional screening of grape rhizosphere soil microorganisms, a biocontrol bacterium with high efficiency of antagonizing grape white rot bacteria is obtained, and basic materials are provided for biological control of grape white rot.

The invention discloses burkholderia gladioli H02, a bacterial agent, a bacterial fertilizer and application of the burkholderia gladioli H02. The strain has a remarkable antagonistic effect on phytopathogens such as sclerotium rolfsii and fusarium oxysporum, has an inhibiting effect on growth of barnyard grass, and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35003. The invention further provides a method for preventing and treating southern blight through combination of H02 and a chemical pesticide thifluzamide for peanut crops. The burkholderia gladioli H02 as well as the microbial agent and the bacterial fertilizer thereof have important significance on control of weed damage, prevention and treatment of soil-borne diseases and pesticide reduction and synergism of agricultural production.

The application relates to a Burkholderia gladioli NDG19 and an application of the Burkholderia gladioli NDG19 in disease resistance, and belongs to the technical field of microorganisms. The Burkholderia gladioli NDG19 is preserved in the Guangdong Provincial Microbial Culture Collection Center on July 31, 2023, the strain preservation number is 63701, and the preservation address is No. 59 Building, 5th Floor, Guangzhou, Guangzhou, China. The Burkholderia gladioli NDG19 provided in the application can effectively inhibit the growth and reproduction of Fusarium oxysporum and Alternaria tenuis, and can effectively prevent and treat plant fusarium wilt such as sharp banana fusarium wilt and melon root rot of cucurbitaceae crops, solves the problems of environmental pollution and food safety caused by chemical fungicides, the strain has the functions of iron carrier production, phosphorus dissolution and potassium dissolution, can effectively improve the utilization rate of phosphorus and potassium in soil as a biological bacterial fertilizer, promotes the growth of plants, is safe and environment-friendly, and has a wide application prospect.

A bacterial enrichment solution and its use in detecting Burkholderia gladioli (Pseudomonas cocovenenans subsp. venom), the bacterial enrichment solution containing the following components: a potato dextrose aqueous solution, gentian violet, chloramphenicol, polymyxin B, and sodium dehydroacetate. The bacterial enrichment solution has a good bacterial enrichment effect on target strains and can effectively inhibit the growth of non-target bacteria that appear frequently during routine detection, reducing interference from non-target bacteria and improving the detection rate and work efficiency of target bacteria. The present invention has a simple preparation process, low cost, and strong operability, and is suitable for enriching Burkholderia gladioli (Pseudomonas cocovenenans subsp. venom) in various foods.

The invention discloses a burkholderia gladioli aptamer and a screening method thereof, and belongs to the technical field of food safety. The DNA (deoxyribonucleic acid) sequence of the burkholderia gladioli aptamer is as shown in SEQ ID No: 1. According to the screening method, computer-aided design is utilized, aptamer candidate sequences highly specifically bound with bacterial membrane protein are rapidly screened from a large-scale nucleic acid sequence library through a molecular docking technology, and the aptamer with excellent affinity and specificity is finally obtained through in-vitro experimental verification and optimization. The method overcomes the defects that a traditional aptamer screening method is long in time consumption, high in cost, complex in operation and the like, and a new way is provided for rapidly and efficiently screening the burkholderia gladioli specific recognition aptamer. The obtained aptamer is combined with fluorescent nano-enzyme for the first time, forms a double-probe structure with concanavalin A (ConA)-loaded magnetic beads, can be used for constructing a burkholderia gladioli rapid detection system, and has a wide application prospect in the fields of food safety monitoring, pathogenic bacterium detection and the like.

The block of natural plant material is subjected to one or more chemical treatments to remove substantially all of the lignin therefrom, thereby allowing the extraction of delignified cellulose fibers. For example, the natural plant material can be a grass, such as bamboo or gladiolus. The extracted fibers are then dried to densify the structure, thereby improving mechanical properties. In some embodiments, the extracted fibers can be used as structural materials alone or in combination with other materials. For example, the extracted fibers can be embedded in a polymer or concrete, infiltrated with a polymer or concrete, coated with a polymer or concrete, or otherwise combined with a polymer or concrete to form a composite material.

A kind of cultivation method for preventing and treating the tip-burning of summer flower bracts of Amaryllis, wherein when the flower sword of Amaryllis is about 14-18 cm long, the whole plant is sprayed with mixed agent I of nano-kaolin, nano-montmorillonite and polyalkylene modified heptamethyltrisiloxane, and after 3-4 days, the whole plant is sprayed with mixed agent II of 25% double acylic acid amide, 5% emamectin benzoate and kaolin; one day before harvesting, the whole plant is sprayed with mixed agent III of 25% double acylic acid amide, 5% emamectin benzoate and polyalkylene modified heptamethyltrisiloxane.The present application improves the resistance of flower bracts of Amaryllis to high temperature and strong light, reduces the occurrence of tip-burning of flower bracts, and improves the quality of cut flowers of Amaryllis in summer cultivation.

The invention discloses a construction method of a growth prediction model of burkholderia gladioli in soaked black fungus and application of the constructed model, the method comprises the following steps: inoculating burkholderia gladioli containing rifampicin resistance into a soaked black fungus sample, putting the soaked black fungus sample into a plurality of incubators at different temperatures, and culturing; calculating the colony count of rifampicin-resistant burkholderia gladioli, and determining the growth curve of the burkholderia gladioli; respectively fitting the three first-level models and the two second-level models at different temperatures by utilizing statistical analysis software; by comparing comprehensive evaluation parameters, an optimal primary model and an optimal secondary model are selected, SGompertz is screened out as the optimal primary model, a quadratic polynomial model is screened out as the secondary model, the models are applied to prediction of the growth condition of burkholderia gladioli in soaked black fungus, the shelf life of the black fungus and the quantitative microbial risk can be predicted, and the quality guarantee period of the black fungus can be predicted. And the food quality can be improved.

The application discloses a Burkholderia glumae RS-36 and application thereof, and belongs to the technical field of microorganisms. Burkholderia gladioli The application provides a Burkholderia glumae (B. glumae) RS-36 with a preservation number of CCTCC No: M 20221584, and application of the B. glumae RS-36. Burkholderia gladioli The B. glumae RS-36 provided by the application has inhibiting effects on Magnaporthe oryzae, Ustilaginoidea virens, Exserohilum turcicum and Pyricularia grisea, can secrete indole acetic acid for promoting plant growth, can keep good activity in an acidic environment with a pH of 5 and a drought environment, has strong stress resistance, and has important significance for promoting green and sustainable development agriculture.

The invention discloses a disease prevention and growth promotion type compound microbial agent and application thereof to vegetables, and belongs to the technical field of microbial agents, and the compound microbial agent comprises burkholderia gladioli, bacillus cereus, antibiotic lysobacter and bacillus velezensis. The fertilizer provides nutrient substances and growth factors required by plants, promotes root growth, improves the soil environment and improves the nutrient absorption efficiency. By using natural microorganisms, the use of chemical pesticides and fertilizers is reduced, the environmental pollution is reduced, the diversity of soil microorganisms is improved, and the soil health is recovered. The biological agent disclosed by the invention can increase the yield and quality of crops, improve the economic benefit, improve the appearance and taste of fruits and improve the commodity value. The disease index of downy mildew is reduced by 34.14% compared with that of a control group, the disease index of powdery mildew is reduced by 25.53% compared with that of the control group, the wilt dead plant rate is reduced by 82.66% compared with that of the control group, the yield is increased by 12.37% compared with that of the control group, and the economic benefit is remarkable.

This invention provides an application of 1-aminocyclopropane-1-carboxylic acid as an antibacterial agent. 1-Aminocyclopropane-1-carboxylic acid is a non-protein amino acid. Through plate and tube antibacterial experiments and plant pathogenicity experiments, this invention discovered a new use for 1-aminocyclopropane-1-carboxylic acid: it exhibits inhibitory effects on microorganisms, including *Escherichia coli*, *Burkholderia gladioli*, *Corynebacterium tarda*, *Corynebacterium glutamicum*, and *Saccharomyces cerevisiae*. 1-Aminocyclopropane-1-carboxylic acid significantly inhibits the pathogenicity of *Burkholderia gladioli* HDXY-02 to plants. Therefore, 1-aminocyclopropane-1-carboxylic acid can be used in agriculture as a seed treatment agent at a concentration of 0.1%–1% (w / w); or as an agricultural antibacterial agent at a spray concentration of 0.1%–1% (w / w).

The invention provides a microbial agent and application thereof, the microbial agent contains burkholderia gladioli with the preservation number of CGMCC No.24934 and alcaligenes faecalis with the preservation number of CGMCC No.31941, and the burkholderia gladioli and the alcaligenes faecalis act together, so that the influence of low-temperature stress on crops can be relieved, the invasion of pathogenic bacteria on the crops can be effectively inhibited, and the growth of the crops can be promoted.

The invention provides a method and a device for detecting burkholderia gladioli. The detection method comprises the following steps: providing a protein sample of a to-be-detected strain, and performing mass spectrum detection to obtain a mass spectrum peak map of the to-be-detected strain; if the mass spectrum peak map contains a first characteristic peak, the to-be-detected bacterial strain comprises bacterial strains except for the toxin-producing bacterial strain; if the mass spectrum peak map contains a second characteristic peak, the to-be-detected bacterial strain comprises a toxin-producing bacterial strain; the mass-to-charge ratio of the first characteristic peak is 7207 Da to 7211 Da, and the mass-to-charge ratio of the second characteristic peak is 7716 Da to 7721 Da. The invention provides a method for rapidly and efficiently detecting whether burkholderia gladioli is a virulent strain or not.

This invention relates to *Burkholderia gladioli* and its application in the preparation of products for controlling fungal diseases in sugarcane, belonging to the field of biological control technology. The *Burkholderia gladioli* of this invention is *Burkholderia gladioli*. Burkholderia gladioli Strain B1, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 37420, is effective in controlling fungal diseases such as sugarcane smut, sugarcane top rot, sugarcane brown streak, and sugarcane red rot. It also possesses phosphorus-solubilizing and growth-promoting properties, thereby effectively promoting nutrient absorption and plant growth in crops.

This invention relates to the field of microbial testing technology, specifically to the application of a specific molecular target for *Burkholderia gladioli* cocovenenans pathogenic species. The specific molecular target for *Burkholderia gladioli* cocovenenans pathogenic species is used to detect *Burkholderia gladioli* cocovenenans pathogenic species. The nucleotide sequence of the specific molecular target for *Burkholderia gladioli* cocovenenans pathogenic species is shown in SEQ ID No. 1 or SEQ ID No. 2. In specificity evaluation, the specific molecular target for *Burkholderia gladioli* cocovenenans pathogenic species exhibits greater coverage of both target and non-target bacteria, enhancing reliability and accuracy.

The invention discloses a burkholderia gladioli strain LB-03 and application of the burkholderia gladioli strain LB-03 to prevention and treatment of sugarcane brown streak, and relates to the technical field of microorganisms. The preservation number of the burkholderia gladioli strain LB-03 disclosed by the invention is GDMCC (China General Microbiological Culture Collection Center) No.62952. The burkholderia gladioli LB-03 provided by the invention can be prepared into a microbial fungicide, is used for preventing and treating sugarcane brown streak, can effectively inhibit the occurrence of the sugarcane brown streak, has no phytotoxicity to sugarcanes, and is environment-friendly. The burkholderia gladioli LB-03 disclosed by the invention is a biological prevention and control strain with potential significance in prevention and control of sugarcane brown streak disease.

The invention discloses burkholderia gladioli LJ04 and application of the burkholderia gladioli LJ04 in antagonism of durian disease pathogenic bacteria, and relates to the technical field of microorganisms, and the preservation number of the burkholderia gladioli LJ04 is GDMCC No: 67473. The strain LJ04 disclosed by the invention has a very strong inhibition effect on the epidemic disease pathogenic bacteria of the elaeagnus wilsonii, and can obviously inhibit the growth of the hypha of the epidemic disease pathogenic bacteria of the elaeagnus wilsonii, the plate inhibition rate of the LJ04 bacterial liquid on the epidemic disease pathogenic bacteria of the elaeagnus wilsonii within 7 days reaches 100%, and the control effect of the LJ04 bacterial liquid reaches 96.57% after the LJ04 bacterial liquid is inoculated for 5 days. As a disease-resistant strain, the burkholderia gladioli LJ04 has very good potential of preventing and treating plant diseases caused by the durian blight pathogenic bacteria, provides an environment-friendly, simple and effective way for preventing and treating the plant diseases caused by the durian blight pathogenic bacteria, and is beneficial to environmental protection.

The present invention discloses a gladiolus Burkholderia (Burkholderia gladioli) HM-72 and its application, which has been deposited in China Center for Type Culture Collection on July 13, 2023, and the deposit address is Wuhan University, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province, and the deposit number is CCTCCNO: M20231291. The strain has strong resistance to Pb stress, has partial phosphate dissolution, and the ability to produce siderophores. After applying the strain in Pb-contaminated soil, the mobility of Pb can be increased, the removal of Pb in the soil is accelerated and accumulated in the above-ground parts of enriched plants, to achieve the problem of repairing soil lead pollution, while the bacteria can inhibit the growth of pathogenic fungi, and its growth-promoting antibacterial properties can also provide favorable conditions for the growth of enriched plants in high-lead environments, promote its biomass accumulation, and have application prospects in environmental remediation of heavy metal pollution.