The present invention belongs to the technical field of
image processing, and discloses a method, a
system and a device for locating all the spotting points of a microfluidic
biochip. The method includes: acquiring the
grayscale source image after spotting on the
biochip, storing it in a specified
directory on the disk, loading it into the memory for subsequent
processing, obtaining a first processed image with the spotting area of the magnetic head separated from the circuit background, and generating a template
grayscale image; performing
template matching on the first processed image by using the
normalized correlation matching method to obtain a result matrix dstmat; then through multi-target positioning
processing, obtaining the positions of the centers of each magnetic head, and recording them in a
list or an array; circularly acquiring the coordinates of the centers of each magnetic head in the
list or the array, judging whether there are abnormal coordinate points, and determining whether the
chip performs subsequent defect detection. The present invention can perform subsequent defect detection on the microfluidic
biochip faster and more accurately, and provides an
effective method for realizing the automatic detection of the quality of the spotting
chip.