The invention belongs to the technical field of flos chrysanthemi processing, and particularly discloses a processing method of lemon and flos chrysanthemi juice wine. The method comprises the following steps: (1), weighing the following raw materials in parts by weight: 29 to 31 parts of fresh flos chrysanthemi petals, 24 to 26 parts of fresh lemon, 940 to 960 parts of corn wine, 17 to 19 parts of fresh dragon fruit peel, 1.2 to 1.4 parts of egg white powder, 0.4 to 0.6 part of chitosan, and 13 to 15 parts of Chinese date honey; (2), obtaining flos chrysanthemi syrup; (3), obtaining primary lemon and flos chrysanthemi juice wine; (4), obtaining lemon and flos chrysanthemi juice wine; (5), filling, performing sterilization, and labeling, so as to obtain a finished product. The processed lemon and flos chrysanthemi juice wine is orange-yellow, clear and transparent, does not contain suspended solids, has the fragrance of flos chrysanthemi, ensures that fragrance of flos chrysanthemi, honey and wine is harmonious, makes body refreshed and fragrant, has light flos chrysanthemi flavor, is moderate in sweet and sour degrees, mellow in vinosity, comfortable and harmonious, complete in wine body, and outstanding in flavor, and has obvious treatment effects on patients suffering from long-term constipation, insomnia and dreaminess, and xerosis cutis.