The invention discloses a fresh water culture pond with a greenhouse structure. The fresh water culture pond comprises a body, the body comprises a culture pond body and a greenhouse, supporting rodsare movably arranged on the inner walls of the two sides of the culture pond body, a transverse moving air cylinder is arranged on the surface of one side of the culture pond body, a piston rod is arranged at the output end of the transverse moving air cylinder, and sliding grooves are formed in the surfaces of the two sides of the top of the culture pond body. By arranging sliding plates, the sliding grooves, the air cylinder, the piston rod and a telescopic rod, the piston rod is driven to stretch out through the air cylinder, so that the greenhouse can slide along the sliding grooves in theculture pond body through the sliding plates, the position is convenient to adjust, and the sliding plates are detachably connected with the piston rod and the telescopic rod; a folding plate is arranged at the side ends of the sliding plates, a piece of inlet baffle cloth is arranged on one side of the greenhouse, and when the greenhouse completely covers the culture pond body, the inlet bafflecloth can be opened, and the folding plate is lifted up, so that the culture personnel can enter the greenhouse conveniently, and can stand on the folding plate, therefore, the culture personnel can conveniently carry out culture operation.