The invention discloses an active light-emitting film, an active light-emitting signboard and a preparation method thereof, and aims to solve the technical problems that an existing active light-emitting signboard is complex in structure and difficult to manufacture, and resources cannot be reasonably utilized. The active light-emitting film comprises a substrate, an LED array, a scattering layer,fluorescent powder glue and a retro-reflection layer, the LED array is arranged on the substrate, the scattering layer is fixed above the LED array, the space between the substrate and the scatteringlayer is filled with the fluorescent powder glue, and the retro-reflection layer is arranged on the side face, back on to the LED array, of the scattering layer; and the active light-emitting sign comprises a support, a signboard panel, a bottom film and an active light-emitting film, the signboard panel is fixedly connected with the support, the bottom film is adhered to the front surface of thesignboard panel, and the active light-emitting film is adhered to the bottom film. The active light-emitting film is directly pasted on an existing signboard, the size is reduced, manufacturing is convenient, and brightness is uniform.