The invention belongs to the field of fluid velocity field measurement, and relates to a tracer particle sowing device for two-phase flow field PIV measurement and an operation method. The problem that tracer particles cannot be effectively sowed in a two-phase flow field PIV measurement experiment at the same time in the prior art is solved. The device comprises a cylindrical pressure holding tank body, a tank body with a mixer, and a lifting particle release. The cylindrical pressure holding tank body comprises a first observation window, an air inlet, a safety valve, a pressure gauge, a first valve, an air outlet and a first hose. The tank body with the mixer comprises a first motor, a water inlet, a second observation window, the mixer, a second valve, a second hose and a water outlet.The lifting particle release comprises a second motor, a screw, a support frame, a connecting rod, a top steel plate, a horizontal pipe and a vertical circular pipe. The device provided by the invention has the advantages of wide application, simple operation and high reliability.