The embodiment of the invention discloses an under-microscope radio frequency scalpel for surgery. The scalpel comprises a tool assembly, a handle assembly, a biopsy assembly, an electronic endoscopeand an illumination assembly. The handle assembly comprises a body and a handle; the tool assembly is arranged at the front end of the body of the handle assembly, the biopsy assembly is arranged at the rear end of the body of the handle assembly, and the electronic endoscope and the illumination assembly are arranged at the front end of the handle of the handle assembly. The scalpel can be applied to a general surgery department, a thoracic surgery department, a hepatological surgery department, a neurosurgery department, a head and neck surgery department, a bone surgery department, an ear-nose-throat surgery department, a urinary surgery department, an anorectal surgery department, a breast surgery department, a pediatric surgery department, an abdomen surgery department, a pancreas surgery department, a digestion surgery department, a vascular surgery department, an endocrine surgery department, a plastic surgery department, an oncology department, a beautifying department, a burnsdepartment, a clinical lab, a pediatric department, a gynecology department and other departments. Tissue suction, biopsy sampling, illumination, normal saline injection, low-frequency direct currentstorage, electronic endoscopy and other functions in surgery can be achieved.