The invention discloses apocynum venetum fiber blended antibiosis, cool and comfortable yarn and a preparing method thereof. The apocynum venetum fiber blended antibiosis, cool and comfortable yarn comprises, by weight, 15-30 percent of apocynum venetum fiber, 35-50 percent of aloe viscose and 35-45 percent of milk fiber. The performance indexes of the blended yarn comprise the single-yarn breaking strength ranging from 11.5 cN/tex to 21.2 cN/tex, the single-yarn breaking strength variable coefficient Cv value ranging from 7.9 percent to 17.0 percent, and the yarn evenness variable coefficient Cv value ranging from 13.5 percent to 19.6 percent. The apocynum venetum fiber blended antibiosis, cool and comfortable yarn has the advantages that the fabric made of the blended yarn has the healthcare functions of antibiosis, antiphlogosis, skin protection, body building, face beautifying, skin moistening and the like, the fabric is high in strength, good in wet absorbing and breathable performance and dyeing performance, soft in hand feeling, good in moistening performance and mold-proof and moth repellency and wide in product usage range, and the fabric can be used for manufacturing various clothes, bedclothes, underwear, sportswear, fitness clothes and other various knitted or woven healthcare textiles.