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Fuel combustion catalyst microburst aerosol delivery device and continuous and consistent aerosol delivery device

Efficient fuel combustion catalyst delivery device and method are provided. A fuel combustion catalyst delivery device includes a storage vessel that contains a liquid. The liquid includes one or more catalytic materials. The storage vessel is attached to a nebulizer that can convert the liquid to an aerosol to be delivered into an air intake of a combustion zone. The aerosol can accelerate fuel combustion. The storage vessel and the nebulizer are made from materials suitable to be exposed to the liquid and the aerosol. The storage vessel and the nebulizer are also suitable to be placed in proximity to the combustion zone such as an engine compartment so that the aerosol can be delivered into the air intake of the combustion zone easily and effectively. The surfaces of the delivery device exposed to the liquid or the aerosol may be made of corrosion-resistant or inert materials if the liquid is acidic. The surfaces of the delivery device exposed to the liquid or the aerosol may be also made from materials that produce substantially no contaminants into the liquid. The nebulizer may convert the liquid to an aerosol at a rate between once per second and one hundred times per second. The nebulizer may have holes for passing the liquid where each of the holes has a size not greater than about 10 μm. The delivery device can deliver up to 500 ml or more of liquid as aerosol over 500 hours of operation.
Owner:EMISSIONS TECH

Methods for amplifying hepatitis c virus nucleic acids

A method of amplifying an HCV nucleic acid in an HCV infected sample comprises amplifying a segment of a DNA template that is complementary to a genome of HCV RNA from the sample by a two-stage PCR, wherein a first stage PCR employs a first outer primer and a second outer primer, and a second stage PCR employs a first inner primer and a second inner primer. The nucleotide sequence of the first outer primer comprises a nucleotide sequence as set forth in SEQ ID NO: 2; or SEQ ID NO:9, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 9. The nucleotide sequence of the second outer primer comprises a nucleotide sequence set forth in SEQ ID NO: 3 or 4; or a nucleotide sequence as set forth in SEQ ID NO: 10 or 11, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 10 and 11. The nucleotide sequence of the first inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 5; or SEQ ID NO:12, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 12. The nucleotide sequence of the second inner primer comprises a nucleotide sequence as set forth in SEQ ID NO: 6 or 7; or a nucleotide sequence as set forth in SEQ ID NO: 13 or 14, wherein optionally 1, 2 or 3 nucleotides are other nucleotides than those of SEQ ID NO: 13 and 14.
Owner:VERTEX PHARMA INC
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