The invention relates to a cascaded Mach-Zehnder interferometer based reconfigurable comb filter and a preparation method thereof. The filter comprises a planar lightwave circuit (PLC) chip, a first optical fiber array and a second optical fiber array, wherein the PLC chip comprises a silicon substrate, a silicon dioxide buffer layer is arranged on the silicon substrate, a waveguide is arranged on the silicon dioxide buffer layer, a coverage layer is arranged on the waveguide, and the waveguide comprises an input-stage Y branch, a first-length fixing arm, a second-length fixing arm, a first opening arm, a second opening arm, a third opening arm, a fourth opening arm, a first-stage 3dB coupler, a second-stage 3dB coupler and an output-stage 3dB coupler. The method comprises the steps of preparing the buffer layer and the waveguide on the silicon substrate so as to obtain a PLC chip portion, slicing and grinding the PLC chip portion, then, coupling the PLC chip portion with the optical fiber arrays, and finally, connecting corresponding optical fibers. The filter and the method have the advantages of reconfigurability, fine adjustable phase, flat-crest filtering and low-nonlinearity output.