The invention relates to a sterile cultivation device capable of achieving automatic monitoring and regulation of multiple culture conditions. The device mainly comprises a cultivation device body, a water inlet, a water outlet, a water storage tank, a wastewater tank, a belt filter pellicle water manifold interface, a multi-interface water suction pump, a water manifold interface, a CPU microprocessor, an oxygen/carbon dioxide monitor, a thermosensitive sensor and other detection instruments, a PH instrument, a pressure sensor, an ion concentration detector and a moisture sensor, wherein the oxygen/carbon dioxide monitor, the thermosensitive sensor, the PH instrument, the pressure sensor, the ion concentration detector and the moisture sensor are connected with the CPU microprocessor through sensor line channels respectively, the water storage tank and the wastewater tank are connected with the cultivation device body into a whole and communicated with a water guiding hose through the multi-interface water suction pump, and the water guiding hose is connected with a filtering and degerming unit; a sterile environment in the cultivation device is guaranteed, whether a plant growth environment is normal is monitored continuously, and normal PH of culture solution in the water storage tank is guaranteed. By the adoption of the device, automatic cultivation can be achieved, long-term cultivation of aseptic seedlings is achieved, and operation is easy and convenient.