The invention discloses a traditional Chinese medicine preparation for treating cute red eyes, sore pain and difficultly-opened eyes with bitter tears and a preparation method of the traditional Chinese medicine preparation. The traditional Chinese medicine preparation comprises the following main raw materials: fructus viticis, flos eriocauli, radix stephaniae tetrandrae, rhizoma anemarrhenae, radix scutellariae, raw radix rehmanniae, radix bupleuri, angelica sinensis, semen celosiae, radix saposhnikoviae, semen cuscutae, silkworm shit, rhizoma cimicifugae, radix platycodonis, radix paeoniae alba, fructus arctii, semen cassiae, rhizoma alismatis and liquorice, wherein the medicinal materials can be prepared into a clinically required finished product preparation by virtue of the preparation process. The traditional Chinese medicine preparation can be used for treating eye diseases such as the cute red eyes, the sore pain and the difficultly-opened eyes with bitter tears and has treatment advantages that the treatment effect is good, both symptoms and root causes are treated, and the relapse is nearly avoided; furthermore, the traditional Chinese medicine preparation has the technical advantages that the preparation process is simple, convenient and feasible, individual difference is small, the pesticide effect is stable and definite, and the traditional Chinese medicine preparation is easy to popularize and apply.