42 results about "Method engineering" patented technology

The present invention a provides methods for production of heterologous polypeptides using a variety recombinantly engineered secretory cell lines. The common feature of these cell lines is the absence of expression of at least one endogenous polypeptide. The host cell machinery normally used to produce the endogenous polypeptide is then usurped for the purpose of making the heterologous polypeptide. Also described are methods engineering cells for high level expression, methods of large scale protein production, and methods for treatment of disease in vivo using viral delivery systems and recombinant cell lines.

The invention discloses a GIS (Geographic Information System) fault diagnosis system and method based on vibration signal spectrum analysis, belonging to the field of GIS fault monitoring. The system comprises a vibration acceleration sensor, a charge amplifier, a data acquisition instrument and a PC (Personal Computer) all of which are successively connected together in series, wherein the vibration acceleration sensor comprises three vibration sensors which are fixed on the outer surface of a GIS box in the X-axis, Y-axis and Z-axis directions, respectively. The diagnosis method of the system comprises the following steps of: acquiring GIS vibration signals in the X-axis, Y-axis and Z-axis directions by means of the three vibration sensors; performing wavelet de-noising on the vibration signals intercepted by a full period and then performing spectrum analysis; calculating energy at 50 Hz and the sum of energy at 100 Hz, energy at 200 Hz and energy at 300 Hz respectively; comparing the calculated values with the normal state; and determining the GIS fault according to the result of comparison in combination with a threshold. The method is easy in engineering realization, obvious in feature and capable of effectively diagnosing the GIS fault.

The invention discloses an amplitude phase error correction system and an amplitude phase error correction method for a transmitting channel of a phased-array antenna. The amplitude phase error correction system comprises a correction signal source, a rotary selector switch, a to-be-corrected transmitting channel, an auxiliary receiving channel and a correction coefficient calculating module. When the amplitude phase error correction is carried out on an n transmitting channel, a correction signal source transmits a correction signal y (k), and the auxiliary receiving channel receives a signal yn (k) at the same time. A correction coefficient calculating module calculates a correction weight signal wn through the signal yn (k) and a reference signal yref (k) in such a way of adopting a minimum mean square self-adaptive filtering algorithm. According to the method and system provided by the invention, the methods engineering can be realized simply, and the correction accuracy is higher, therefore the system and the method can be applied to the field of the amplitude phase error correction of a uniform circular array phased-array antenna.

The invention discloses an online monitoring method for a tunnel internal pipeline. The method comprises the step that a pipeline strain monitoring portion, a pipeline and environment temperature monitoring portion, a support displacement monitoring portion, a pipe clamp pressure monitoring portion and a remote control portion are included; the pipeline strain monitoring portion is used for measuring axial strain of the pipeline; the pipeline and environment temperature monitoring portion is used for measuring running temperature of the pipeline and environment temperature inside a tunnel; the support displacement monitoring portion is used for measuring relative displacement between a support bottom plate and a tunnel ground foundation; and the pipe clamp pressure monitoring portion is used for monitoring pipe clamp pressure changes. The online monitoring method can achieve continuous monitoring of the tunnel internal pipeline and transmits safety conditions of the tunnel internal pipeline, the support and the pipe clamp to a remote analysis terminal in real time. The method facilitates engineering application, and the safety management level of a tunnel penetrating pipeline can be improved.

The invention provides an artificial top construction method based on paste filling and belongs to the technical field of mining engineering. The method comprises the steps of drift excavation, beam head excavation, hanging bar arrangement and paste filling. According to the method, artificial top filling drifts are excavated firstly, and a steel bracket and a timber support are erected; secondly, chambers are excavated on the two sides of a roadway every other 3 m, and each chamber is 150 cm wide, 150 cm height and 200 cm deep; thirdly, bar arrangement is conducted, bottom bars and hanging bars are arranged, and the bottom bars include horizontal bars and longitudinal bars; fourthly, paste filling is conducted, strength reaches 4-5 Mpa within 28 days, and the mass concentration of paste is 78-80%. According to the method, work amount is small, construction cost is low, construction is easy, risks caused by insufficient span or excessive span of a filled artificial top are avoided, drilling safety is improved for workers, and high-level large-diameter deep-hole safe and efficient recovery of weak broken ore bodies can be achieved. The method provides a new idea for large-scale mining of the ore bodies.

The invention discloses a method for remediating contaminated underground water in situ on basis of a persulfate thermal activation technique, which relates to a method for remediating contaminated underground water. The method is used to solve the problems of large investment and low efficiency of the conventional underground water remediation technique. The method comprises: adding persulfate or persulfate compound oxidizer into water, and pumping the solution by an anticorrosion pump into underground water to be remediated; and 2, after the persulfate or persulfate compound oxidizer is mixed with the underground water for 10 to 24 hours, pumping high-pressure vapor into the underground water to be remediated, and remediating the contaminated underground water after 1 to 2 days. When the method disclosed by the invention is used, the work amount is small, the technical investment is small, new toxic matters are not introduced into water, the implementation is easy, and the removal rate of the contaminants reaches 85 to 95 percent. The method is used in the field of underground contaminated water treatment.

The invention discloses a light and convenient electromagnetic high-energy dynamic compaction device and method and an application. In the light and convenient electromagnetic high-energy dynamic compaction device, an electromagnet assembly is arranged above an outer sleeve, an impact rod is arranged in the outer sleeve, an impact hammer assembly is arranged under the outer sleeve, multiple levels of electromagnetic accelerating coils are arranged on the inner lateral wall of the outer sleeve and respectively connected with circuit control systems externally, and a hook ring is arranged at the top of the impact rod. By means of the method, the impact rod is accelerated through the electromagnetic effect of the multiple levels of electromagnetic accelerating coils during the process that the impact rod falls down to strike the impact hammer, and accordingly the impact energy and speed can be improved. The device is applied to foundation reinforcing in civil engineering, the total weight of the device is less than 1/10 of the weight of a traditional dynamic compaction device with the same compaction energy, the occupied space is greatly reduced, the problem of heavy construction devices, difficult transportation, limited construction speed, high cost, high energy consumption, limited device using space and the like of construction devices in dynamic consolidation method engineering application can be well solved or improved, and the efficient, environment-friendly, safe and wide application can be promoted.

The invention discloses an interval-type radar radiation source signal feature type recognition capability analyzing method. The method comprises the following steps: describing distribution ranges and probabilities of interval-type signal features of different types of radar radiation sources through a histogram; comparing distribution differences of the interval-type signal features of the different types of radar radiation sources on the basis; further analyzing interval-type signal feature type recognition capabilities; comparing the interval-type signal feature type recognition capabilities; and outputting optimal interval-type signal features of the different types of radar radiation sources. The method has the advantages that: (1) a method for describing the interval-type radar radiation source signal features through the histogram is proposed, so that the limitation in description of a single fixed data value through a conventional illustrative diagram is broken; (2) based on histogram description, the optimal interval-type features of the radar radiation sources can be further analyzed and recognized; and (3) the calculation cost is low, and method engineering is realized.

The invention discloses a probability technology based thermal protection system design method. The probability technology based thermal protection system design method comprises the steps of firstly building a parameterized model of a thermal protection system in consideration of random distribution of thermal random variables; then generating various random input samples by utilizing an efficient sampling technology; then carrying out transient thermal analysis on samples in probability analysis and regarding a temperature characteristic quantity at one position and at one moment of the thermal protection system in a flying process as an output variable; then building a polynomial response surface model on the basis of a small amount of existing samples; and finally, carrying out Monte Carlo direct sampling on the response surface model to obtain probability distribution of the temperature characteristic quantity of the thermal protection system. According to the probability technology based thermal protection system design method, probability design of the thermal protection system is realized, a system reliability index and input random variable sensitivity are obtained, and a direction for scheme design and optimization of the thermal protection system is provided. In addition, the calculation efficiency is greatly improved through combination of the efficient sampling technology and a numerical integration algorithm; and the method engineering provided by the invention is available.

The invention discloses a photovoltaic cell power control system and method capable of automatically adapting to load demand changes. The photovoltaic cell power control system comprises a photovoltaic cell controller, wherein the photovoltaic cell controller controls the output power of a photovoltaic cell. Current sensors collect currents. The method comprises the steps that firstly, the photovoltaic output current and the load current are collected through the current sensors, then, the relation between the output power of the photovoltaic cell and the load demand of the photovoltaic cell is judged through the collected currents, and finally, the photovoltaic cell controller controls the output power of the photovoltaic cell according to the relation. According to the photovoltaic cell power control system and method capable of automatically adapting to the load demand changes, the relation between the output power of the photovoltaic cell and the load demand of the photovoltaic cell is obtained through the photovoltaic output current and the load current, using is flexible, the judging method engineering for the load demand is easy to implement, and impact on the network voltage is small.

The invention relates to an underground coal mine stress field principal stress direction predicting method. The method is mainly characterized by including the steps that a regional stress field type is preliminarily predicted according to the regional structure evolution history and the modern structure movement characteristics; crossed tunnels with the basically same supporting type in the same stratum are selected for further judging and observing the regional stress field type; the maximum horizontal principal stress direction of the stress field at an observation site is judged according to the underground stress trace geometrical characteristics; through the combination of the stress field type and the maximum horizontal principal stress direction, the maximum principal stress direction, the intermediate principal stress direction and the minimum principal stress direction of the stress field are judged. By means of the method, the problems that an existing coal mine stress field testing method is high in engineering cost and complicated in process are solved; by means of the relations of tunnel deformation, stress trace characteristics, stress field type and principal stress direction, the economical, practical and effective underground coal mine stress field principal stress direction predicting method is put forward.

The present method is a method to automate the repeatible process of application engineering microprocessor based control systems and creating the related documentation to submit for engineer approval and construct the control system. Prior to this method engineering every decision about point assignment, part selection, controller addressing et al was either made and only recorded upon the engineering documents or made in the field and either recorded manually or not at all. As the result when those engineering decisions were required elsewhere in the engineering documents individuals could only refer to those documents when retabulating the information. The present method allows a user to record all most all of the engineering decisions in a soft environment allowing the decisions to be repeatably used in every engineer document. As the result efficiency in the task of application engineering is increased and errors created as information is transferred are eliminated.

The invention relates to an in-orbit fuel consumption balancing method of a formation satellite system. The method comprises the following steps that 1) after kth formation control, the fuel consumption Ek of the formation control is calculated; 2) after the kth formation control, a formation control parameter [delta]lk of two satellite orbit periods is determined; 3) the sum SEk of cumulative fuel consumption of formation control and a residual error [delta]lk of present formation control are substituted into a formula SEk>=k1&&[delta]lk>=k2 for determination; and 4) if the formula is valid, marks of main and auxiliary satellites are switched autonomous on the satellites, main and auxiliary satellite switching control is carried out via a ground annotation instruction or on satellites autonomously so that a new main satellite operates in a reference orbit, parameters are set as initial values, a new auxiliary satellite executes a formation control task, and the steps 1) to 3) are repeated; or if the formula is invalid, the original auxiliary satellite continues to carry out formation control. The method is high in engineering realization performance, can be used to save fuels, requirements of formation satellite tasks are taken into consideration, and the autonomous level of the satellites can be improved.

The invention discloses an engineering reentry guidance method oriented to a moving mass aircraft. The engineering reentry guidance method is used for solving the technical problem of poor engineeringapplicability of an existing moving mass aircraft reentry guidance method. According to the engineering reentry guidance method, on the basis of the most common proportional guidance law in the engineering, the landing velocity and the landing angle constraint of the moving mass aircraft is converted into an augmented item, the augmented item parameter is further subjected to robustness expandingdesign, so that the guidance parameter is a function of the flight state deviation of the moving mass aircraft, the guidance precision is ensured when the moving mass aircraft is interfered, and finally the guidance instruction, namely the roll angle instruction size, is determined on the basis of the required overload and the available overload relation. According to the method, on the basis ofa proportional guidance law, the augmented item of the landing angle and the landing speed constraint is added, trajectory lifting and reduction are achieved, meanwhile, the landing angle and the landing velocity constraint are met, and meanwhile, the robustness expanding design is carried out on the augmented term coefficient on the basis, and the method is a moving mass aircraft strong robust guidance method which is simple in form and good in engineering application performance.

The invention relates to a protection technology of a cold region dispersive soil side slope, in particular to a protection method of the cold region dispersive soil side slope. The protection method aims to solve the problems that frost heaving, stability losing and erosion damaging are prone to occurring in the cold region dispersive soil side slope, and an existing protection method is large in project amount and large in rain wash influence. The protection method comprises the steps that 1 a layer of geotechnical cloth is pre-laid at the position where a composite material is to be laid; 2 a polyurethane binder and gravel are mixed evenly to obtain the composite material; 3 the composite material obtained in the step 2 is laid on a channel side slope before solidification, wherein the laying thickness D satisfies the equation (please see the formula in the specifications), Hz refers to the effective wave height, R equals to 0,35, delta refers to the relative density of a slope protection layer, refers to a wave breaking parameter and is calculated according to the equation (please see the formula in the specifications), refers to the average wave length and alpha is the slope protection inclination; 4 a catch drain is excavate in the top of a channel; 5 the composite material obtained in the step 2 is laid in the foot fixing position at the bottom of the channel, and then protection of the cold region dispersive soil side slope is completed.

The invention provides a method for determining an acting range of a biogas gas guiding well of a municipal solid waste landfill, comprising the following steps of: drilling a plurality of gas guiding wells in the municipal solid waste landfill along the same straight line; selecting the two gas guiding wells to be respectively used as a gas pumping well and a detection well; if the pressure of the detection well is positive pressure, determining that the acting range of the gas guiding wells is less than the distance between the two wells; if the pressure of the detection well is negative pressure, determining that the acting range of the gas guiding wells is more than the distance between the two wells; selecting different gas guiding wells to be combined to gradually and accurately determine the acting range interval of the gas guiding wells; and when the pressure of the detection well is zero or weak negative pressure and the oxygen concentration is more than zero, determining that the distance between the detection well and the gas pumping well is the acting range of the gas guiding wells. According to the method for determining the acting range of the biogas gas guiding well of the municipal solid waste landfill, the problems of the existing method that the engineering period is long and the quantity of the drilled wells is more are solved, and the aim of determining the acting well of the gas pumping well by an optimized scheme is achieved; and the method is convenient to operate and is safe and practical.

The invention relates to a multi-section combined earthwork vertical lifting device. A bottom section of a structure framework is mounted on a foundation pit excavating surface; a standard section of the structure framework is mounted on the bottom section; a top section of the structure framework is mounted on the standard section; a supporting wheel is mounted on the structure framework; guide wheels are further mounted on the bottom section and the top section; track chains are mounted in the grooves of the supporting wheel and the guiding wheels; a hopper is mounted on a hopper shaft through a hinge; the hopper shaft is mounted between two track chains; a plurality of driving devices are mounted on a beam plate or a trestle of a top layer structure and beam plates of all structures in the lower layer, and are used for driving the track chain to drive the hopper to be lifted; a ratchet device is mounted at the bottom of the hopper; opening and closing control is performed through the ratchet device, so that unloading is accomplished. The device solves the problems that rotation operation is required when a long arm excavator or a caterpillar grab bucket shovel beside a soil outlet performs vertical lifting soil loading in reverse building method engineering, and large operating space is required, greatly improves the operating efficiency of earthwork soil discharging in the reverse building method engineering, and meanwhile reduces the space occupied by oil discharging operation.

The invention discloses an updating method of arid desert area populus diversifolia root searching and cutting. The method comprises the following steps of 1, updating area selection; 2, setting updating time; 3, predicting a populus diversifolia root system trend and selecting a reasonable root cutting position; 4, time-delay backfilling. Compared with an existing annular groove updating technology, the updating method has the following advantages that project updating implementation time is set, and the situations are avoided that operation is carried out in a strong wind season, a rooting cutting pit is buried by sand and the advantage conditions of populus diversifolia rooting cutting sprouting seedling are broken; at the same time, because a new sand source is formed by man-made soil disturbance, the strong wind season is avoided and sandy oil amount carried by wind erosion is reduced; the updating position is selectively determined, and the density of a populus diversifolia forest is reasonably distributed; the project amount of the method is decreased by over 78% than annular groove updating, and manpower and material resources are saved; the method is simple, easy to implement and master, quick in returns, good in effect and strong in practicability and operability.

The invention discloses a frequency stepping signal speed compensation system based on difference frequency double waveforms. The frequency stepping signal speed compensation system comprises a difference frequency dual-waveband signal generating unit (2), a broadband antenna feed system (3), a difference frequency dual-waveband signal receiving unit (4), a frequency source (5) and a signal processor (6); the difference frequency dual-waveband signal generating unit (2) transmits a difference frequency dual-waveband signal; the difference frequency dual-waveband signal receiving unit (4) receives an echo signal; the signal processor (6) implements speed compensation. The frequency stepping signal speed compensation system disclosed by the invention can solve the problem of poor engineeringrealizability of the conventional stepping frequency signal speed measurement method, is realized through hardware design and is less in condition constrain, strong in engineering realizability and high in compensation precision.

The invention discloses a main track traffic-keeping method used during the construction period of highway reconstruction and extension. According to the method, two temporary openings are successively formed in each segment of median strip; a highway to be reconstructed and extended is divided into construction areas A and construction areas B in a long and short alternate mode; after one construction area A is constructed to a lower layer under the road surface first, vehicles run in the corresponding construction area B through the temporary openings of the median strip; and the positions of the passing temporary openings are changed according to the construction progress, so that working faces are created for alternate construction of the construction areas A and the construction areas B, and whole-time two-way four-lane traffic-keeping is realized. By means of the method, the engineering sense is clear, and operation is easy. The method can be used for two-way four-lane traffic-keeping of a main track during the construction period when a two-way four-lane highway is reconstructed and extended into eight two-way lanes or more.

The invention discloses a refining method for seal oil. The seal oil is purified by adopting a molecular distillation method. The distillation temperature is low, the system vacuum degree is high, and the materials are heated for a relatively short time, so that oxidative decomposition of effective components in a processing process can be effectively prevented, and high-throughput stable operation can be maintained. The defects of complex procedures, great labor intensity, incontinuous production, organic chemical residual and the like of the existing methods are overcome, and through synthesizing polyunsaturated fatty acid glycerides by using microorganism lipase under a water system environment, absorption and digestion by the human body are further facilitated. The seal oil disclosed by the invention is rich in various polyunsaturated fatty acids such as EPA, DHA, DPA and the like, is relatively good in color and taste indexes, and can be used for health-care applications such as fatty liver and prostatic hyperplasia prevention and treatment and the like.