The invention discloses an optimal design method of a phased array feed source based on focal plane field sampling of a reflector antenna, relates to a high-performance multi-beam antenna technology in the fields of communication, measurement and control, radio astronomy and the like, and aims to provide a phased array feed source design method which is high in efficiency, low in channel number and high in cost performance. According to the method, the characteristics of the focal plane field of the reflector antenna at different focal diameter ratios and different scanning angles are analyzed, the parameter selection principle of the phased array feed source during optimal sampling of the focal plane field is studied, the relation between the antenna aperture efficiency and the PAF design parameters is summarized, a relation curve of the optimal unit spacing of the phased array feed source and the focal diameter ratio is given, and the accuracy of the phased array feed source is improved. A calculation formula of the number of PAF units is exported, a PAF design example and performance analysis are given, and the effectiveness of the design method is verified. The method is suitable for the design of feed sources in the fields of radio astronomy, satellite communication, deep space exploration and the like.