The invention provides a novel micro-fluidic chip forming die and method and belongs to the field of micromachining. The die mainly comprises a forming assembly, a demoulding assembly, a die assembling air cylinder, a fixing bracket, M5*15 hexagonal head bolts, guide pillars, a guide pillar connection plate, M5*30 hexagonal head bolts, a temperature controller, a movable die plate, a table, a die jacking air cylinder, a jacking rod push plate, a core die fixing block, jacking rods, spring sleeves, a copper pressing block, a core die and a direct current power source. The novel micro-fluidic chip forming method includes the steps that die assembling is performed, wherein liquid-state dimethyl siloxane is poured into a die cavity; the direct current power source is utilized to heat the surface of the die cavity; product solidification is performed, wherein after die sinking is performed, the demoulding assembly jacks a product out; and charging bars and flanges are removed, and the product is obtained. The novel micro-fluidic chip forming method has the advantages of being energy saving, safe and high in forming precision.