The invention provides a novel micro-fluidic 
chip forming die and method and belongs to the field of micromachining. The die mainly comprises a forming 
assembly, a demoulding 
assembly, a die assembling air cylinder, a fixing bracket, M5*15 hexagonal head bolts, guide pillars, a guide pillar connection plate, M5*30 hexagonal head bolts, a temperature controller, a movable die plate, a table, a die jacking air cylinder, a jacking rod push plate, a core die fixing block, jacking rods, spring sleeves, a 
copper pressing block, a core die and a 
direct current power source. The novel micro-fluidic 
chip forming method includes the steps that die assembling is performed, wherein liquid-state 
dimethyl siloxane is poured into a die cavity; the 
direct current power source is utilized to heat the surface of the die cavity; product solidification is performed, wherein after die sinking is performed, the demoulding 
assembly jacks a product out; and charging bars and flanges are removed, and the product is obtained. The novel micro-fluidic 
chip forming method has the advantages of being energy saving, safe and high in forming precision.