The invention discloses a traditional Chinese medicine compound composition with effect of relieving mental fatigue. The traditional Chinese medicine compound composition is prepared from the following raw materials in parts by weight: 35 to 65 parts of acer truncatum buge oil nervonic acid, 5 to 9 parts of silybum marianum protein, 15 to 25 parts of rana japonica oil, 3 to 7 parts of radix valerianae officinalis , 2 to 5 parts of flos melissae officinalis, 1 to 3 parts of radix ranunculi ternati, 1 to 3 parts of okra, 1 to 3 parts of purple daisy flower, 1 to 3 parts of chamomile flower and 1to 3 parts of propolis, and is prepared by the following steps of crushing the dried radix valerianae officinalis , flos melissae officinalis, radix ranunculi ternati, okra, purple daisy flower and chamomile flower for further use; heating and dissolving the rana japonica oil and the propolis in preparation equipment, and cooling for 3 to 5min; respectively adding the acer truncatum buge oil nervonic acid, the silybum marianum protein and the powder of radix valerianae officinalis , flos melissae officinalis, radix ranunculi ternati, okra, purple daisy flower and chamomile into the preparation equipment, stirring until completely dispersing, standing in the environment with temperature lower than or equal to 30 DEG C and relative humidity of 45 to 60%, and cooling to normal temperature of18 to 25 DEG C, so as to obtain the composition. The traditional Chinese medicine compound composition has the advantages that the safety and effectivity are realized, the obvious function of relieving mental fatigue is realized, and the functions of regulating central nerves, protecting heart and cerebral vessels, calming stress and the like are realized.