The invention discloses a preparation method of a peanut sprout sauce. The preparation method comprises the following steps of (1) collecting peanut sprouts, performing thorough cleaning, performing blanching with boiling water, taking out the blanched peanut sprouts, quickly placing the taken peanut sprouts in cold water for soaking, and immediately exchanging water until the temperature of the peanut sprouts is reduced to normal temperature; (2) placing the peanut sprouts of which the temperature is reduced in rice washing water for soaking for 1-5 days, taking out the soaked peanut sprouts,performing thorough cleaning, performing draining, and performing dicing to obtain diced peanut sprouts; (3) taking fresh chicken meat, and performing dicing to obtain diced chicken meat; and (4) taking vegetable oil, performing heating to 80-90 DEG C, adding Chinese prickly ash, star aniseeds, garlic and table salt, performing frying until fragrance is obtained, then adding the diced chicken meat, performing stir-frying with an intense fire, until 90% of the diced chicken meat is cooked, then adding the diced peanut sprouts, performing cooking for 2-5min, performing taking out, performing loading in cans, performing sealing and performing sterilizing, wherein the weight ratio of the vegetable oil to the Chinese prickly ash to the star aniseeds to the garlic to the table salt to the dicedchicken meat to the diced peanut sprouts is (15-30) to (4-8) to (5-10) to (3-6) to (2.5-4) to (20-30) to 100. The peanut sprout sauce is refreshing and crisp in mouth feel, has delicious and fragranttaste of the chicken meat, is easy to carry and delicious, can be eaten with rice, and is a leisure food suitable for old people and young people.