The invention discloses a biltong which comprises pork, duck, fish meat, salt, white sugar, gourmet powder, fructus amomi, cassiabarktree fruit, myrcia, radix angelicae, ginger, pericarpium citri reticulatae, cinnamon, amomum tsao-ko, Chinese prickly ash and comb; the invention further discloses a preparation method of the biltong; the preparation method comprises the following steps: selecting material, cleaning, disacidifying, sterilizing, adding condiment, and smudging. The biltong provided by the invention not only can conform to an eating habit of Chinese, and but also can meet requirements of appearance, nutrition and safety of export to meat product, and meanwhile, the preparation method of the biltong is not limited by seasons, environment and temperature, so that industrialized and large-scale industrial production can be formed.