The invention discloses a work fixture for machining inner cavities and joint closing surfaces of glass molds, and belongs to the technical field of work fixtures. The work fixture comprises a bottom seat plate, a middle seat plate, a movable seat, a fixed seat, a traction screw of the movable seat and mold clamping mechanisms; a first adjusting groove, a second adjusting groove and a third adjusting groove are arranged on one side of the bottom seat plate; the middle seat plate is positioned in the center of the bottom seat plate, one end of the middle seat plate is fixed to the first adjusting groove, and the other end of the middle seat plate is fixed to the second adjusting groove; the movable seat is arranged at one end of the middle seat plate, the fixed seat is fixed to the other end of the middle seat plate, an inclined plane of the movable seat is formed on one side of the movable seat, an inclined plane of the fixed seat is formed on one side of the fixed seat, and the inclined plane of the movable seat and the inclined plane of the fixed seat form a V shape; one end of the traction screw of the movable seat is connected with the movable seat, and the other end of the traction screw of the movable seat is supported on the fixed seat; and one mold locking mechanism is positioned on one side of the middle seat plate, the other mold locking mechanism is positioned on the other side of the middle seat plate, and the mold locking mechanisms are respectively fixed to the third adjusting groove. The work fixture has the advantages that the work fixture is simple in structure, convenient to manufacture, quick in operation and good in clamping effect, and tends to be maintenance-free, and loosening is prevented during machining.