The invention discloses a lead-acid storage battery SOH estimation method based on SA and ANN algorithms. The method comprises the steps: S1, carrying out cyclic charging and discharging experiment ona lead-acid storage battery, and recording the one-to-one correspondence between the actual capacity and the number of cycles of the cyclic charging and discharging experiment, and the time of constant-voltage charging and constant-current charging in a charging stage of each experiment; S2, based on the test result of the step S1, determining an influence factor with the highest SOH associationdegree with the lead-acid storage battery, and establishing an artificial neural network regression model; and S3, training weights and bias values in the artificial neural network regression model through an improved simulated annealing algorithm, establishing a new regression model, and estimating the battery capacity data points of the SOH of the lead-acid storage battery by using the new regression model. According to the invention, the artificial neural network model ANN is adopted, the method has the advantages of being good in nonlinear approximation capacity and generalization performance, small in number of training samples and high in fitting precision, the number of model parameters is small, and the calculation speed is high.