The invention relates to a high-accuracy temperature compensation crystal oscillator system and an operational method of the high-accuracy temperature compensation crystal oscillator system. The high-accuracy temperature compensation crystal oscillator system comprises a computer, a power supply, an incubator, a frequency meter, a frequency-scaling and data-transporting and displaying unit, a data input and output (DIO) card used for driving the testing controlling board arranged on the computer mainboard in an inserting mode, and a general purpose interface bus (GPIB) card used for driving the incubator, the frequency meter, and the power supply. Software based on event-driving controls the power supply, the incubator and the frequency meter through the DIO card and the GPIB card, reads multiple sets of data of each set temperature point and stores the data inside a computer database. The multiple sets of data are synthesized into a plurality of five-order functions in a set temperature range, and a best curve is calculated by the software. Each coefficient of the best curve is extracted, and is converted into numbers inside the an internal memory of a crystal oscillator chip through an analog to digital (A/D) converter, and therefore the number of capacitors controlled by the memory is changed, and the purpose of high-accuracy temperature compensation of the crystal oscillator is achieved. The high-accuracy temperature compensation crystal oscillator system and the operational method of the high-accuracy temperature compensation crystal oscillator system can be produced in scale and are high in working efficiency.