The invention relates to a terahertz non-bianisotropic metamaterial unmarked sensor, and a preparation method and a use thereof, and belongs to the biotechnical field. The unmarked sensor comprises aflexible substrate polyimide, a metal layer is arranged on the flexible substrate polyimide, the metal layer comprises a lower titanium metal layer having a thickness of 20 nm and an upper gold metallayer having a thickness of 200 nm, the titanium metal layer is arranged on the flexible substrate polyimide, and the metal layer is a metal resonance ring with two openings. The asymmetric metal resonance ring with two openings is designed to realize electromagnetic induction of a transparent formant, the surface of a metamaterial is inoculated with cells through cell culture, the resonance frequency shift of a device is detected in a back transmission manner, and the theoretic sensitivity reaches up to 455 GHz/RIU, so the concentration of cancer cells can be rapidly preliminarily detected inan unmarked manner, and the apoptosis trend of oral cancer cells HSC3 under different anticancer drug action times is obtained.