The invention belongs to the technical field of satellite network communication, and discloses a Walker constellation-oriented Mobius constellation topological configuration construction method, system and application. According to the implementation scheme, the method comprises the following steps: initializing parameters of Walker constellations and satellite antenna link establishment constraints, and numbering all satellites; according to the constellation parameters and the link establishment constraints, calculating a link establishment set of each satellite; searching a link establishment mode capable of establishing a Mobius constellation topological structure in the different-orbit link establishment set, and generating a link combination set; and establishing an in-orbit link andan out-of-orbit link of the Walker constellation according to the link combination set, and constructing a Mobius constellation topological structure. According to the method, the average path hop count of the satellite network is shortened, the connectivity of the network is enhanced, the average end-to-end delay of the network is reduced, and the information bearing capacity of the Walker constellation space segment is improved. According to the invention, a topology structure with optimal connectivity of the Walker constellation in a one-satellite four-chain mode is provided, the routing cost is reduced, and the cost performance of the network is improved.