The invention relates to a biopharmaceutical stirring device, which comprises a base, a fixing rod, a fixed box, a stirring mechanism and a sealing mechanism, the stirring mechanism comprises a stirring assembly and a driving assembly, and the sealing mechanism comprises a sealing assembly and a connecting assembly. According to the biopharmaceutical stirring device, primary stirring of a main body is achieved through rotation of a connecting rod around a rotating rod, irregular movement of a stirring unit is achieved through a first spring, secondary stirring of the main body is achieved, and through cooperation of a first gear and a second gear, a connecting rod rotates along the axis of a bearing; the rotation of a collision plate is realized, the three-stage stirring of the main body is realized, the stirring effect is improved, and the sealing of the fixed box is realizedthrough the sealing mechanism, the fixed box is sealed through a sealing mechanism, people are prompted that the main body is being stirred through rotation of a circular plate, a sealing plate is limited through restoring force of a second spring, the sealing plate is prevented from being separated from the fixing box, and linkage of a plurality of mechanisms is achieved through the same driving source, and the use practicability is improved.