The invention discloses an electronic tongue detection device for meat stuffing pastry production and a detection method, the electronic tongue detection device comprises a fixed seat, a storage cavity is formed in the outer wall of one side of the fixed seat, box doors are connected to the inner walls of the two opposite sides of the storage cavity through hinges, and a fixed groove is formed in one side of the outer wall of the top of the fixed seat; a fixing groove is formed in the inner wall of the bottom of the storage cavity, a rotating disc is arranged on the inner wall of the bottom of the fixing groove, a plurality of detection grooves are formed in the outer wall of the top of the rotating disc, a driving mechanism is arranged in the storage cavity, a fixing mechanism is arranged in the detection grooves and comprises an air bag and an air cushion, an air pipe is arranged between the air cushion and the air bag, and the air bag is annular. According to the device, the fixing effect of the fixing mechanism on the container can be conveniently improved, the situation that meat stuffing cake samples are scattered from the interior of the container due to shaking, and consequently detection of meat stuffing cakes is affected is prevented, heat dissipation treatment can be conducted on work of the device, and lubricating treatment can be conveniently conducted between tooth grooves and gears.