The invention discloses an automatic seed tray filling and cleaning device for biological breeding. The automatic seed tray filling and cleaning device comprises a tray filling and placing device body, a cross rod is fixed in an inner cavity of the tray filling and placing device body, a supporting plate is fixed to the surface of one side of the tray filling and placing device body, a water tank is arranged on the surface of the top end of the supporting plate, a liquid guide pipe penetrates through the inner wall of one side of the water tank, the liquid guide pipe penetrates through the outer wall of one side of the tray filling and placing device body, the side, away from the water tank, of the liquid guide pipe extends into an inner cavity of the cross rod, and water outlet pipes distributed at equal intervals are fixed to the surface of one side. The water tank is arranged, so that seeds in a placing box can be conveniently and quickly cleaned, and the cultivation quality of the seeds in the later period is guaranteed; a liquid storage box is arranged, so that sewage after cleaning is filtered and then recycled, and the utilization rate of water is increased; and through a reinforcing sleeve seat, the joint of the liquid guide pipe and the water tank can be reinforced conveniently, the flow guide effect of the liquid guide pipe on water is guaranteed, and the sealing performance of the device is improved.