The invention discloses an evidence-based method for screening gastric cancer prognosis molecular markers. The method comprises the steps as follows: 1), an inclusion criterion is set for a gastric cancer prognosis biomarker experimental research; 2), a research evidence retrieval strategy is set; 3), the prognosis biomarkers are screened preliminarily; 4), research data are extracted; 5), quality assessment on the experimental research quality is performed; 6), standardized data are processed; 7), the prognosis biomarkers are screened; and 8), according to the screening criterion, the gastric cancer prognosis biomarkers having potential application values are screened, and the prognosis values are described. In conclusion, the evidence-based method for screening the gastric cancer prognosis biomarkers is provided, and the gastric cancer prognosis biomarkers are screened, so that the screening cost is reduced, the screening time is shortened, professional thresholds are reduced, and the application values of the prognosis biomarkers are increased.