The invention relates to a testing, adjusting and sealing examining integrated device for a separate injection well. The testing, adjusting and sealing examining integrated device is applied to underground separate layer water injection of an oil field and comprises an underground separate injection tubular column and a testing, adjusting and sealing examining system. The testing, adjusting and sealing examining system comprises an integrated testing, adjusting and sealing examining instrument, and the integrated testing, adjusting and sealing examining instrument comprises a control unit, a flow sensor, an internal pressure sensor, an upper motor, a lead screw, an axial positioning unit, an upper and lower packing unit, an external pressure sensor, a circumferential positioning unit and a rotating unit. The axial positioning unit comprises a positioning block provided with an axial anti-clamping sliding groove, and the lead screw is provided with an upper setting thread and a lower setting thread which are opposite in rotating direction. The flow sensor is an electromagnetic flow sensor. A packing piece is a compression type rubber sleeve or a packing cup. The upper setting thread and the lower setting thread are trapezoidal threads. The two-stage packing element and the setting mechanism of the two-stage packing element are arranged on the integrated testing, adjusting and sealing examining instrument and matched with an efficient water distributor, testing, adjusting, pressure measuring and sealing examining operation can be completed at a time.