The invention relates to a sample purification preparation system, which comprises a purification preparation body, a filter plate is arranged on the purification preparation body in a drawing mode, afixing clamping column is arranged at one end of the filter plate, a clamping groove is formed in the purification preparation body, the fixed clamping column is clamped in the clamping groove, limiting openings are further formed in the two sides of the fixing clamping column, and a fixator is arranged on the side wall of the purification preparation body. The fixator and the limiting opening are arranged in a limiting fit mode. The filter plate and the purification preparation body are mounted, the fixed clamping column is clamped in the clamping groove firstly, then a driving motor is started, a driving bevel gear rotates to drive a driven bevel gear to rotate, a fixed rotating shaft rotates, and a positive thread area and a negative thread area are in threaded connection with the thread blocks, so that the two thread blocks move oppositely; the limiting clamping rods penetrate through the through holes in the purification preparation body to limit the screw blocks, and meanwhile,the two limiting clamping blocks are matched with the limiting holes in a limiting manner to fix the fixed clamping columns; and the angle change of the two buffer hinge rods drives the buffer springto stretch and reset.