The invention discloses a polypeptide acne-removing repair essence liquid and a preparation method thereof. The essence liquid is rich in beautifying polypeptide components and mainly comprises hexapeptide-9, tetrapeptide-30, an antibacterial peptide, a penetrating peptide, aloe barbadensis mill, milk yeast, butanediol, glycerol, sodium hyaluronate, squalane, tocopherol, yellow collagen, stearic acid, lauryl alcohol polyether-3, phenoxyethanol, methylparaben and ethylparaben, wherein the total weight percentages of the four beautifying polypeptides such as the hexapeptide-9, the tetrapeptide-30, the antibacterial peptide and the penetrating peptide is 10-30%. According to the present invention, the four beautifying polypeptides are the main function components of the product, and the research results show that the hexapeptide-9, the tetrapeptide-30 and the antibacterial peptide have strong antibacterial effect and strong acne removing effect while the penetrating peptide has good transdermal effect, such that the facial acne can be rapidly eliminated by using the product and the facial acne mark can be restored after the long-term use of the product.