The invention belongs to the technical field of nipples, and particularly relates to a preparation method of a high-toughness silicone nipple. The method concretely comprises the following steps of (1) weighing raw materials according to the parts by weight; (2) mixing liquid silicone rubber, amino silicon oil, gallic acid, Pulullan, sodium caseinate and sodium carboxymethylcellulose, stirring through a stirrer, and freezing in a freezing tank, so as to obtain a primary mixture; (3) mixing the primary mixture, Arabic gum and water, stirring through the stirrer, and magnetizing in a magnetic field, so as to obtain a secondary mixture; (4) putting the second mixture into a vacuum kneading machine, and vacuum kneading, so as to obtain a mixture; (5) injecting the mixture into a mould throughan injection moulding machine, moulding, and cooling, so as to obtain the high-toughness silicone nipple. The tensile strength, the tearing strength, the elongation and the resilience rate of the nipple are effectively improved, so that the nipple has higher toughness, the damage rate and the tearing rate of the nipple are effectively reduced, the service life of the nipple is prolonged, and the nipple is safe, health and reliable to use.