The invention provides a TCR-T cell for killing tumors, which is characterized in that the TCR-T cell is a T cell carrying a tumor antigen TCR, TCR in the TCR-T cells is derived from any one or more of the following T cells: 1) CD4T cells for expressing one or more of genes such as TNFRSF18, CXCL13, TNFRSF4, TNFSF8, ENTPD1, ACP5, LAYN, TNFRSF9, CTLA4, CD200 and TIGIT in tumors; and 2) a CD8T cellfor expressing one or more of the genes of TNFRSF18, CXCL13, CXCR6, GALNT2, ENTPD1, ACP5, HAVCR2, LAYN, TNFRSF9, CTLA4 and CD109 in the tumor. The TCR-T cell provided by the invention can be effectively applied to treatment of tumors, especially immunotherapy.