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48 results about "Cd4 t cell" patented technology

CD4 cells (also known as CD4+ T cells) are white blood cells that fight infection. The more you have, the better. These are the cells that the HIV virus kills.

Method for inducing CCR5-delta32 deletion with genome editing technology CRISPR-Cas9

PendingCN105567738AWide range of infectionVectorsHydrolasesHIV receptorNatural resistance
The invention relates to a method for successfully inducing cell chemokine receptor CCR5 genes to be mutated into CCR5-delta32 deletion-type genes with a new genome editing technology CRISPR-Cas9. CCR5 is an important receptor for human immunodeficiency viruses (HIV) to invade personal host cells. CCR5-delta32 deletion means deletion of 32 basic groups occurs in a CCR5 coding region, so that the sequence after the 185th amino acid is changed, and early termination occurs. CCR5-delta32 biallelic-gene homozygous deletion has natural resistance to HIV infection, and can not be infected by HIV. By means of the method, a slow virus packaging system and the CRISPR technology are used at the same time; as the slow virus infecting host range is wide, the method can be applied to cells such as bone marrow stem cells and CD4T cells, and the CCR5-delta32 deletion-type genes hopefully become medicine for treating acquired immune deficiency syndrome or other diseases.
Owner:NANKAI UNIV

T cell regulation

Regulatory T cells (Treg) limit autoimmunity but can also attenuate the magnitude of anti-pathogen and anti-tumor immunity. Understanding the mechanism of Treg function and therapeutic manipulation of Treg in vivo requires identification of Treg selective receptors. A comparative analysis of gene expression arrays from antigen specific CD4+ T cells differentiating to either an effector / memory or a regulatory phenotype revealed Treg selective expression of LAG-3 (CD223), a CD4-related molecule that binds MHC class II. LAG-3 expression on CD4+ T cells correlates with the cells' in vitro suppressor activity, and ectopic expression of LAG-3 on CD4 T cells confers suppressor activity on the T cells. Antibodies to LAG-3 inhibit suppression both in vitro and in vivo. LAG-3 marks regulatory T cell populations and contributes to their suppressor activity.
Owner:ST JUDE CHILDRENS RES HOSPITAL INC +1

System and method for producing t cells

InactiveUS20110236363A1Enhance preT cell expansionControl expansionBiocideGenetically modified cellsProgenitorStromal cell
Disclosed herein is a system and method for producing T cells from stem cell populations. Specifically exemplified herein is a culture system and method that produces CD4 cells and / or T cell subtypes from a CD4 lineage using a sample of hematopoietic stem cells. Adult hematopoietic precursor / stem cells (HPC) are progenitors to all lineages of immune cells. There has been limited success in generating functional CD4 T cells with this convenient culture system. Also disclosed herein is a novel stromal cell line expressing DL1, interleukin-7 (IL-7), and FMS-like tyrosine kinase 3 ligand (Flt3-L). This improved culture system can greatly facilitate the study of late T cell development and enables immunotherapeutic applications.
Owner:UNIV OF FLORIDA RES FOUNDATION INC

Novel recombinant poxvirus composition and uses thereof

The present invention provides a recombinant pox virus composition comprising a nucleic acid sequence encoding chemokines as costimulatory molecules. The present invention further provides a host cell, a host animal, and a pharmaceutical composition comprising the recombinant pox virus composition. Also provided is a method for treating or preventing a neoplasm or infectious disease in a subject, using the pox virus composition and / or an SLC agent. Additionally, the present invention provides a method for promoting the proliferation of a CD4 T cell, comprising administering to the cell an SLC agent in an amount effective to directly promote the proliferation of the cell. Finally, the present invention provides a method for treating or preventing a neoplasm or infectious disease in a subject using cultured CD4 T cells.
Owner:THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK

Chimeric human leukocyte antigen and epitope-bearing molecules having immunosuppressant activity

The present invention relates to chimeric molecules that comprise human major histocompatibility complex elements, linked to epitopes of interest, that have been joined together to form dimers or higher multimers. Such chimeric molecules may be used to treat or prevent diseases associated with autoimmunity, particularly diabetes. It is based, at least in part, on the discovery that a chimeric molecule comprising an IILA-DR element linked to an epitope of GAD65, an antigen associated with autoimmune diabetes, stimulated the secretion of the inhibitory cytokine IL-10 from CD4 T cells of Type I diabetic patients.
Owner:MT SINAI SCHOOL OF MEDICINE

HIV pre-immunization and immunotherapy

The present invention relates generally to immunization and immunotherapy for the treatment or prevention of HIV. In particular, the methods relate to in vivo and ex vivo enrichment of HIV-specific CD4 T cells. In certain embodiments, the disclosed compositions and methods can incorporate therapy in order to further enhance the HIV-specific CD4 T cells.
Owner:AMERICAN GENE TECH INT

Methods and compositions for treatment of HIV infection

Methods and compositions for treatment of human immunodeficiency virus (HIV) infections have been developed which dampen immune activation with a bias more on the CD4 T cells relative to the CD8 T cell response, inhibit HIV replication, reactivate latent HIV, and inhibit infection of cells by HIV. Pushing latent HIV into active infections with hindrance of cell infection by the reactivated HIV can substantially reduce the number of cells infected with HIV and the viral load of HIV, which is not achieved using just the combination of ART and compounds which activate latent HIV. The methods involve administering to an HIV-infected subject three or more compounds which collectively dampen immune activation with a bias more on the CD4 T cells relative to the CD8 T cell response, inhibit HIV replication, reactivate latent HIV, and inhibiting infection of CD4 T cells by HIV.
Owner:COOPER HUMAN SYST

Manipulation of PTEN in T cells as a strategy to modulate immune responses

The present invention relates to compositions and methods for modulating the expression and / or activity of PTEN in T cells, regulatory T cells, and CD4 T cells. The manipulation of PTEN provides a means for regulating an immune response.
Owner:THE TRUSTEES OF THE UNIV OF PENNSYLVANIA

Promiscuous pap cd4 t cell epitopes

The present invention relates to the discovery of novel T cell epitopes of the human prostatic acid phosphatase (PAP) protein that is promiscuous for at least 15 different HLA-DR alleles. The invention also relates to compositions that contain one of the novel epitopes or a fusion peptide of such an epitope and a heterologous polypeptide. Further disclosed herein is the use of the epitopes or their fusion peptides, and compositions containing the epitopes or their fusion peptides.
Owner:DENDREON PHARMA LLC

Application of DC-CIK (Dendritic Cell-Cytokine-Induced Killer) cells in preparation of medicine used for resisting HIV (Human Immunodeficiency Virus) infection

The invention belongs to the field of biomedicine and discloses application of DC-CIK cells in preparation of a medicine used for resisting HIV infection. Through a lot of experiments, the inventor discovers that when CIK cells impacted by HIV and DC cells are co-cultured, the CIK cells can obviously promote maturation of the DC cells. When the DC and CIK cells are combined for use, the DC and CIK cells have mutual promotion presentation and a function for killing cells infected by HIV. After 30 days of the DC-CIK cell infusion, the number of CD4T cells of a HIV infector is obviously increased, the killing function of NK (Natural Killer) and CIK cells is enhanced, and HIV viral load is obviously restrained. Thus, kill cells induced by co-cultured dendritic cells and cytokines can be applied to the preparation of the medicine used for resisting HIV infection.
Owner:JIANGSU PROVINCIAL CENT FOR DISEASE PREVENTION & CONTROL

Common epitope, antibody, identification method and application of multiple subtype influenza virus HA2 proteins

ActiveCN111440228AHigh sensitivityShorten the steps to prepare monoclonal antibodiesSsRNA viruses negative-senseViral antigen ingredientsAntigen epitopeWestern blot
The invention relates to a multi-subtype influenza virus HA2 protein common epitope, an antibody, an identification method and application. The antigen epitope can be combined with immune serum of H1,H3, H4, H5, H6, H7, H8, H9 and H10 subtype influenza viruses. The method comprises the following steps: selecting an H5N1 subtype influenza virus strain S, designing 18 polypeptides with the length of 20 amino acids and overlapped 10 amino acids according to the sequence of the HA2 protein of the H5N1 subtype influenza virus strain S, artificially synthesizing, spotting to a modified silica gel film to prepare a polypeptide chip, and screening universal epitopes by using antiserum aiming at different subtype IAVs. The recombinant virus with the mutated epitope is constructed, and the bindingcapacity of the epitope and immune serum is analyzed through western blot. Results show that 485-FYHKCDNECME-495 on the HA2 protein has broad-spectrum binding activity with H1, H3, H4, H5, H6, H7, H8,H9 and H10 subtype IAV antiserum. K / D mutation in the recombinant virus leads to reduction of the serum binding activity, which indicates that the K / D mutation is a key amino acid of the serum binding activity. The peptide fragment also comprises an HLA-DR1 (Human Leukocyte Antigen-DR1) restrictive CD4 T cell epitope.
Owner:YANGZHOU UNIV

Method for treating autoimmune disease using cd4 t-cells with engineered stabilization of expression of endogenous foxp3 gene

Disclosed are methods of making a genetically cell that expressed FOXP3 and methods of treatment. In some embodiments, the method can providing a first nucleotide sequence, wherein the first nucleotide sequence comprises a coding strand, the coding strand comprising one or more regulatory elements and a FOXP3 gene or portion thereof providing a nuclease and performing a gene editing process on the first nucleotide sequence, which edits said one or more regulatory elements, and optionally edits the FOXP3 gene or portion thereof. Methods of treating a subject suffering from an autoimmune disease and subjects suffering the effects of organ transplantation are also provided.
Owner:SEATTLE CHILDRENS HOSPITAL (DBA SEATTLE CHILDRENS RES INST)

TCR-T cell for killing tumors as well as preparation method and application of TCR-T cell

The invention provides a TCR-T cell for killing tumors, which is characterized in that the TCR-T cell is a T cell carrying a tumor antigen TCR, TCR in the TCR-T cells is derived from any one or more of the following T cells: 1) CD4T cells for expressing one or more of genes such as TNFRSF18, CXCL13, TNFRSF4, TNFSF8, ENTPD1, ACP5, LAYN, TNFRSF9, CTLA4, CD200 and TIGIT in tumors; and 2) a CD8T cellfor expressing one or more of the genes of TNFRSF18, CXCL13, CXCR6, GALNT2, ENTPD1, ACP5, HAVCR2, LAYN, TNFRSF9, CTLA4 and CD109 in the tumor. The TCR-T cell provided by the invention can be effectively applied to treatment of tumors, especially immunotherapy.
Owner:GUANGZHOU FINELMMUNE BIOTECHNOLOGY CO LTD

Promiscuous pap cd4 t cell epitopes

The present invention relates to the discovery of novel T cell epitopes of the human prostatic acid phosphatase (PAP) protein that is promiscuous for at least 15 different HLA-DR alleles. The invention also relates to compositions that contain one of the novel epitopes or a fusion peptide of such an epitope and a heterologous polypeptide. Further disclosed herein is the use of the epitopes or their fusion peptides, and compositions containing the epitopes or their fusion peptides.
Owner:DENDREON PHARMA LLC

Biomarkers for the Diagnosis of Autoimmune Disease

Compositions and methods are provided for diagnosis and treatment of rheumatoid arthritis. Defects in T cell receptor signaling lower the activation threshold of RA T cells, thus predisposing for a failure in maintenance of immune tolerance. Overexpression of B-Raf and / or K-Ras in CD4 T cells lowers the threshold to respond to TCR triggering in the absence of costimulation and increases responses to citrullinated peptides and other autoantigens.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV +1

Peptide MHCII Tetramers to Detect Endogenous Calnexin Specific CD4 T Cells

The present application discloses proteins or peptides and methods of using such proteins or peptides to evaluate the immune status of a patient. In one embodiment, proteins or peptides may be used to detect endogenous calnexin specific CD4 T cells. In one preferred embodiment, the proteins or peptides may comprise peptide-MHCII tetramers (pMHC tetramers).
Owner:WISCONSIN ALUMNI RES FOUND

Antibacterial peptide as well as applications thereof to preparation of anti-infective drugs, antitumor drugs, immunopotentiators and feed additives

ActiveCN104098666AHas inhibitory effectNot easy to induce drug resistanceAntibacterial agentsFungiBacteroidesFeed conversion ratio
The invention discloses a plutella xylostella antibacterial peptide as well as applications thereof to preparation of anti-infective drugs, antitumor drugs, immunopotentiators and feed additives. An amino acid sequence of the plutella xylostella antibacterial peptide is represented by SEQ ID No.3. The plutella xylostella antibacterial peptide has inhibition and killing effects on various bacteria, so that the peptide can be used for preparing the anti-infective drugs; the plutella xylostella antibacterial peptide has the extremely significant growth inhibition effect on breast cancer cells, hepatoma cells, colonic cancer cells and lung cancer cells and has the obvious antitumor activity in vitro, so that the peptide can be used for preparing the antitumor drugs; the plutella xylostella antibacterial peptide can promote CD4T cells to proliferate and secrete IFNgamma, can inhibit synthesis of IL4 and regulate balance of Th1 / Th2 and has the immunological enhancement activity, so that the peptide can be used for preparing the immunopotentiators; the plutella xylostella antibacterial peptide can significantly increase the survival rate of piglets, reduce the diarrhea rate of the piglets and growing and fattening pigs, increase the daily gain of the piglets and the growing and fattening pigs and reduce the feed conversion ratio of the piglets and the growing and fattening pigs when being used as the feed additives.
Owner:合肥迈可罗生物工程有限公司

Novel Compounds for Enhancing MHC Class II Therapies

The invention provides classes of novel compounds that accelerate peptide loading to DR in the absence of DM and related pharmaceutical compositions. The invention also provides conjugates of these compounds with peptides, antigens or other MHC-based therapeutics, including peptides that self-catalyze their loading onto MHC Class II molecules. Methods are provided for modulating an immune response in a subject. Also disclosed are methods of using the novel compounds, e.g., in combination with MHC-based therapeutics, for the treatment of autoimmune diseases and for the manufacture of medicaments. Methods of improving loading of viral peptides and tumor peptides for enhancing the CD4 T cell response following vaccination against viruses or tumors, and related vaccines, are also provided.
Owner:DANA FARBER CANCER INST INC +1

Ex-vivo priming for generating cytotoxic T lymphocytes specific for non-tumor antigens to treat autoimmune and allergic disease

Cytotoxic T lymphocytes (CTLs) specific for antigenic peptides derived from IgE molecule can be generated in vitro by stimulating resting naive CD8 T cells with IgE peptides presented by artificial antigen presenting cells. The IgE specific CTLs lyse the target cells loaded with IgE peptides in vitro and inhibit antigen specific IgE response in vivo. In addition, adoptive transfer of the IgE specific CTL to an asthmatic mouse model can inhibit the development of lung inflammation and airway hypersensitivity. IgE specific CTL provides a treatment for allergic asthma and other IgE-mediated allergic diseases. Antigenic peptides identified from non-tumor self-antigens induce specific cytotoxic T lymphocyte (CTL) in vitro. The CTL induced by peptides identified from CD40L can kill activated CD4 T cells. In vitro generated CTL specific for CD40L inhibit CD4-dependent antibody responses of all isotypes in vivo. In contrast, CTL induced by antigenic peptides derived from IgE specifically inhibit IgE responses, and adoptive transfer of CD40L-specific CTL to NOD mice at early age delay the development of diabetes in NOD mice. In vitro generated CTL specific for non-tumor self-antigens expressed on activated CD4 T cells regulate immune responses in vivo.
Owner:ORTHO MCNEIL PHARM INC

CD4 T Cell Vaccine and Use Thereof

The present invention relates to a CD4 T cell vaccine and a use thereof, and provides a CD4 T cell vaccine which can increase intracellular viability and effectively induce an antigen-specific cytotoxic T lymphocyte (CTL) response.
Owner:VIGENCELL INC

Promiscuous her-2/neu cd4 t cell epitopes

The present invention relates to the discovery of novel T cell epitopes of the human HER-2 / Neu protein that is promiscuous for at least 25 different HLA-DR alleles. The invention also relates to compositions that contain one of the novel epitopes or a fusion peptide of such a epitope and a heterologous polypeptide. Further disclosed herein is the use of the epitopes or their fusion peptides, and compositions containing the epitopes or their fusion peptides.
Owner:DENDREON PHARMA LLC

Novel coronavirus compound vaccine based on DNA vector and replicating vaccinia virus vector

The invention provides a recombinant vaccinia virus capable of simultaneously expressing an extracellular segment of a new coronavirus S gene and a full-length segment of an N gene, and also provides application of an immunization kit containing the recombinant vaccinia virus, a DNA carrier capable of expressing the extracellular segment of the new coronavirus S gene and a DNA carrier capable of expressing the full-length segment of the N gene of the new coronavirus as a composite vaccine. According to the invention, experimental animals can be induced to generate specific CD4 and CD8 cell reactions aiming at new coronavirus N, S1 and S2 proteins by sequentially inoculating the three vaccines in the composite vaccine provided by the invention in 0, 2 and 4 weeks, the CD8T cell reaction is higher than the CD4T cell reaction, the induced T cell immunity can last for at least 3 months, and the combined antibody lasts for 6 months and is not obviously reduced, so that tt is shown that the composite vaccine can induce lasting immune response to SARS-CoV-2, and a neutralizing antibody induced by the vaccine has cross reaction activity and can prevent infection of various variants of the new coronavirus to a certain extent.
Owner:NAT CENT FOR AIDSSTD CONTROL & PREVENTION CHINESE CENT FOR DISEASE CONTROL & PREVENTION

Vaccine for use in prophylaxis and/or treatment of disease

The present invention relates to an adenoviral vector capable of encoding a virus-like particle (VLP), the VLP displaying an inactive immune-suppressive domain (ISD). The vaccine of the invention shows an improved immune response from either of both of the response pathways initiated by CD4 T cells or CD8 T cells.
Owner:盈珀治疗有限公司

Ex-vivo priming for generating cytotoxic t lymphocytes specific for non-tumor antigens to treat autoimmune and allergic disease

Cytotoxic T lymphocytes (CTLs) specific for antigenic peptides derived from IgE molecule can be generated in vitro by stimulating resting naive CD8 T cells with IgE peptides presented by artificial antigen presenting cells. The IgE specific CTLs lyse the target cells loaded with IgE peptides in vitro and inhibit antigen specific IgE response in vivo. In addition, adoptive transfer of the IgE specific CTL to an asthmatic mouse model can inhibit the development of lung inflammation and airway hypersensitivity. IgE specific CTL provides a treatment for allergic asthma and other IgE-mediated allergic diseases. Antigenic peptides identified from non-tumor self-antigens induce specific cytotoxic T lymphocyte (CTL) in vitro. The CTL induced by peptides identified from CD40L can kill activated CD4 T cells. In vitro generated CTL specific for CD40L inhibit CD4-dependent antibody responses of all isotypes in vivo. In contrast, CTL induced by antigenic peptides derived from IgE specifically inhibit IgE responses, and adoptive transfer of CD40L-specific CTL to NOD mice at early age delay the development of diabetes in NOD mice. In vitro generated CTL specific for non-tumor self-antigens expressed on activated CD4 T cells regulate immune responses in vivo.
Owner:ORTHO MCNEIL PHARM INC
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