The invention relates to a multi-subtype influenza
virus HA2
protein common
epitope, an
antibody, an identification method and application. The
antigen epitope can be combined with immune serum of H1,H3, H4, H5, H6, H7, H8, H9 and H10 subtype influenza viruses. The method comprises the following steps: selecting an H5N1 subtype influenza
virus strain S, designing 18 polypeptides with the length of 20 amino acids and overlapped 10 amino acids according to the sequence of the HA2
protein of the H5N1 subtype influenza
virus strain S, artificially synthesizing, spotting to a modified
silica gel film to prepare a polypeptide
chip, and screening universal epitopes by using
antiserum aiming at different subtype IAVs. The
recombinant virus with the mutated
epitope is constructed, and the bindingcapacity of the epitope and immune serum is analyzed through
western blot. Results show that 485-FYHKCDNECME-495 on the HA2
protein has broad-spectrum binding activity with H1, H3, H4, H5, H6, H7, H8,H9 and H10 subtype IAV
antiserum. K / D
mutation in the
recombinant virus leads to reduction of the serum binding activity, which indicates that the K / D
mutation is a key
amino acid of the serum binding activity. The
peptide fragment also comprises an HLA-DR1 (
Human Leukocyte Antigen-DR1) restrictive CD4
T cell epitope.