The invention relates to a wireless mobile real-time acquisition device for electroencephalograph of small animals, and belongs to the technical field of wireless transmission. The device comprises an electrode, an electroencephalograph acquisition module, an RF wireless transmission module, a power supply module and a PC receiving module, wherein the electrode comprises two dual-lead channels and a reference electrode; and the RF wireless transmission module comprises a second synchronous serial interface and a wireless radio frequency transmitter. The wireless mobile real-time acquisition device has the advantages of small volume, light weight, low power consumption, convenient and accurate actual acquisition of electroencephalograph signals of the small animals, capability of performing real-time electroencephalograph acquisition on the small animals in the active state, suitability for long-term and uninterrupted electroencephalograph monitoring, low cost and high applicability.