This application provides a
nanoprobe-based electrophysiological
signal and biochemical biomarker detection
system, comprising: a patch-clamp
amplifier; a
working electrode electrically connected to a
signal acquisition module, the surface of which, except for the tip, is covered by an insulating protective layer, forming a GΩ-level impedance seal after implantation into the
cell; a counter
electrode connected to a BATH port, with its active end placed in the matrix environment of the target
cell, forming a
current loop; a
reference electrode connected to a REF port, with its active end placed in the matrix environment of the target
cell to provide a potential reference point; the patch-clamp
amplifier is configured to operate in
voltage-clamp mode, with the REF port connected to the
reference electrode as a
voltage sensing terminal, and the BATH port connected to the counter
electrode as a current output terminal, simultaneously acquiring the electrophysiological
signal of the target cell and the current signal corresponding to the concentration of the target biochemical biomarker. This application provides a powerful analytical tool for a deeper understanding of the electrochemical signal
coupling mechanism of neurons.